| Field | Specification |
|---|---|
| Target | |
| Alternative names | Hamartin; Tuberous sclerosis 1 protein; TSC1; KIAA0243; TSC |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Hamartin |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 23T33 is a rabbit monoclonal antibody that recognizes hamartin (gene TSC1) in human samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 129.8 kDa, and the supplier reports an observed band at about 150 kDa.
Hamartin (TSC1) partners with tuberin (TSC2) and TBC1D7 in the TSC complex, which switches off the small GTPase RHEB and thereby restrains mTORC1, a central regulator of cell growth. TSC1 stabilizes TSC2 within this complex, also acts as a co-chaperone of HSP90, and is a tumor suppressor whose mutation causes tuberous sclerosis complex.
| Target | Hamartin (gene TSC1; UniProt Q92574, human) |
|---|---|
| Clone | 23T33 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 129.8 kDa |
| Observed MW | 150 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:1000–1:5000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:200 |
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human hamartin.
Reactivity Notes
The supplier lists reactivity with human. UniProt places hamartin in the lysosome membrane and cytoplasm. Tissue expression noted by UniProt (human): Highly expressed in skeletal muscle, followed by heart, brain, placenta, pancreas, lung, liver and kidney. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
