| Field | Specification |
|---|---|
| Target | |
| Alternative names | Hamartin;Tuberous sclerosis 1 protein;TSC1;KIAA0243, TSC; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human Hamartin recombinant protein (Position: D686-Y884). Human Hamartin shares 96% and 95% amino acid (aa) sequences identity with mouse and rat Hamartin, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets hamartin (gene TSC1) in human and rat samples and is validated for Western blot. The predicted molecular weight is 129.8 kDa, and the supplier reports an observed band at about 130 kDa.
Hamartin (TSC1) partners with tuberin (TSC2) and TBC1D7 in the TSC complex, which switches off the small GTPase RHEB and thereby restrains mTORC1, a central regulator of cell growth. TSC1 stabilizes TSC2 within this complex, also acts as a co-chaperone of HSP90, and is a tumor suppressor whose mutation causes tuberous sclerosis complex.
| Target | Hamartin (gene TSC1; UniProt Q92574, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 129.8 kDa |
| Observed MW | 130 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Rat) |
|---|
Samples with a confirmed band (WB): rat brain tissue, human HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human Hamartin fragment (Asp686–Tyr884), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 96% and 95%, respectively.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places hamartin in the lysosome membrane and cytoplasm. Tissue expression noted by UniProt (human): Highly expressed in skeletal muscle, followed by heart, brain, placenta, pancreas, lung, liver and kidney. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Lihua Ren et al. (2019) Silica nanoparticles induce spermatocyte cell autophagy through microRNA-494 targeting AKT in GC-2spd cells. Environmental Pollution. 10.1016/j.envpol.2019.113172
