| Field | Specification |
|---|---|
| Target | |
| Alternative names | Proheparin-binding EGF-like growth factor; Hbegf; Dtr; Hegfl |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived mouse Hbegf recombinant protein (Position: D63-L148). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets proheparin-binding EGF-like growth factor (gene Hbegf) in mouse and rat samples and is validated for Western blot and ELISA. The predicted molecular weight is 22.8 kDa, and the supplier reports an observed band at about 20 kDa.
Heparin-binding EGF-like growth factor (HB-EGF, HBEGF) is an EGF family ligand made as a membrane-anchored precursor that is shed to release a soluble growth factor. It binds EGFR and ERBB4, and it is needed for normal heart valve formation. The membrane form is also the cellular receptor for diphtheria toxin. HB-EGF is studied in wound healing, heart development and cancer.
| Target | Proheparin-binding EGF-like growth factor (gene Hbegf; UniProt Q06186, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 22.8 kDa |
| Observed MW | 20 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse, Rat) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): rat heart tissue, mouse heart tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant mouse Hbegf fragment (Asp63–Leu148), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with mouse and rat. Tissue expression noted by UniProt (mouse): Most abundant in kidney, skeletal muscle, lung, spleen, brain and heart. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
