| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | hBUB1, BUB1A |
| Clonality | |
| Concentration | |
| Formulation | |
| Immunogen | Purified ascites. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Shipping | |
| Storage |
Product Overview
Research Use OnlyThe BUB family of serine/threonine kinases is a major target of oncology therapeutic research and development. hBUB1 and hBUBR1 are kinases that associate with hBUB3 and are essential components of the mitotic checkpoint. hBUBR1 appears to provide two checkpoint functions by monitoring CENP-E-dependent activities at the kinetochore and by regulating cyclosome/APC activity.hBUBR1 is required in order for microtubule inhibitors to arrest cells in mitosis.
Validated Applications
| Western Blot (WB) | See datasheet for recommended dilution |
|---|---|
| Immunoprecipitation (IP) | See datasheet for recommended dilution |
| Immunofluorescence (IF/ICC) | See datasheet for recommended dilution |
Key Specifications
| Clonality | Monoclonal |
|---|---|
| Species Reactivity | Human |
| Isotype | IgG1 |
| Concentration | 100ug/100ul |
| Formulation | Liquid |
| Catalog No. | Y120001 |
| Alternative Names | hBUB1, BUB1A |
Immunogen / Antigen
Purified ascites.
Reactivity Notes
This antibody has been validated for reactivity against Human targets. Cross-reactivity with other species may exist but has not been formally established.
Additional Information
Application Notes: Titrate for optimal dilution for your application.
Storage & Handling
This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Safety
For Research Use Only (RUO). Not intended for diagnostic, therapeutic, or veterinary use. Handle according to standard laboratory biosafety guidelines. Avoid skin and eye contact. Dispose of waste in accordance with local regulations.
This antibody has been validated for: Western Blot, Immunoprecipitation, Immunofluorescence. For each application, follow the recommended dilutions on the product datasheet and optimize empirically for your sample type.
Titrate for optimal dilution for your application.
This antibody has been confirmed reactive against: Human. Cross-reactivity with other species may occur due to sequence homology but is not guaranteed without independent validation.
Purified ascites.
This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt. Avoid repeated freeze-thaw cycles. Centrifuge vials briefly before opening. Dilute immediately before use.
Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
