| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Histone deacetylase 10; HD10; HDAC10 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E. coli-derived human HDAC10 recombinant protein (Position: T3-Q290). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of HDAC10 (Long-chain-fatty-acid--CoA ligase 5) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-HDAC10 Antibody Picoband® catalog # A05215-2. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E. coli-derived human HDAC10 recombinant protein (Position: T3-Q290). (reported region: T3-Q290).
- Molecular weight context: reported MW: 71 kDa; calculated MW: 39411 MW
- Reactivity: Human,Mouse,Rat
- Applications: ELISA, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Long-chain-fatty-acid--CoA ligase 5; histone deacetylase 10. Histone deacetylase 10 is an enzyme that in humans is encoded by the HDAC10 gene. The protein encoded by this gene belongs to the histone deacetylase family, members of which deacetylate lysine residues on the N-terminal part of the core histones. Histone deacetylation modulates chromatin structure, and plays an important role in transcriptional regulation, cell cycle progression, and developmental events. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Functional note: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Reported localization: Cytoplasm. Nucleus. Excluded from the nucleoli. Expression/tissue context: Ubiquitous. High expression in liver, spleen, pancreas and kidney.
Research relevance and current trends
- Acetylation: Researchers commonly examine how HDAC10 (Long-chain-fatty-acid--CoA ligase 5) relates to this theme using model systems and orthogonal readouts.
- Chromatin Modifying Enzymes: Researchers commonly examine how HDAC10 (Long-chain-fatty-acid--CoA ligase 5) relates to this theme using model systems and orthogonal readouts.
- Class I: Researchers commonly examine how HDAC10 (Long-chain-fatty-acid--CoA ligase 5) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative HDAC10 (Long-chain-fatty-acid--CoA ligase 5) levels across conditions; band patterns may reflect isoforms and processing.
- ELISA-compatible use: when applicable, interpret signal as relative abundance across sample sets with consistent handling and dilution strategy.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
