| Field | Specification |
|---|---|
| Target | |
| Alternative names | Histone deacetylase 10;HD10;3.5.1.98;HDAC10; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human HDAC10 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone HEO-8 is a rabbit monoclonal antibody that recognizes polyamine deacetylase HDAC10 in human and mouse samples and is validated for Western blot, ICC/IF and immunoprecipitation. The predicted molecular weight is 71.4 kDa, and the supplier reports an observed band at about 75 kDa.
HDAC10 is a class IIb deacetylase that works mainly as a cytosolic polyamine deacetylase, removing acetyl groups preferentially from N8-acetylspermidine. Histone deacetylase activity has been seen in vitro, but its physiological importance is uncertain. HDAC10 is studied in polyamine metabolism, autophagy and cancer.
| Target | Polyamine deacetylase HDAC10 (gene HDAC10; UniProt Q969S8, human) |
|---|---|
| Clone | HEO-8 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 71.4 kDa |
| Observed MW | 75 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:1000–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
Samples with confirmed staining (ICC/IF): HeLa cells.
Immunogen
Synthetic peptide based on the sequence of human HDAC10.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt places polyamine deacetylase HDAC10 in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): Widely expressed with high levels in liver and kidney. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
