| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Histone deacetylase 10;HD10;3.5.1.98;HDAC10; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human HDAC10 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-HDAC10 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone HEO-8; isotype Rabbit IgG; reactivity: Human,Mouse. Reported application contexts include WB, ICC, IF, IP (as provided in the source record). Boster Bio Anti-HDAC10 Rabbit Monoclonal Antibody catalog # M05215. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human, Mouse.
Key elements and design rationale
- Target: HDAC10 (Histone deacetylase 10).
- Antibody format: Monoclonal; clone HEO-8; isotype Rabbit IgG.
- Host: Rabbit.
- Species reactivity: Human,Mouse (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
HDAC10 (protein: T-cell surface glycoprotein CD3 zeta chain) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Reported cellular localization context: Cytoplasm. Nucleus. Excluded from the nucleoli. Tissue expression notes (as provided): Ubiquitous. High expression in liver, spleen, pancreas and kidney.
Research relevance and current trends
- Research context keywords from the source record include: Acetylation,Chromatin Modifying Enzymes,Class I,Epigenetics and Nuclear Signaling,Signaling Pathways,Stem Cells.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
- Immunoprecipitation (IP): enrich target complexes for downstream immunoblot or interaction analyses.
Workflow ideas (metafield): Validate HDAC10 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect HDAC10 expression by Western blot in cell or tissue lysates, Localize HDAC10 by immunofluorescence/immunocytochemistry in cultured cells, Enrich HDAC10 by immunoprecipitation from lysates for downstream analysis
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 17 kDa; calculated MW: 71445 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 17 kDa
- Cellular localization (provided): Cytoplasm. Nucleus. Excluded from the nucleoli.
- Tissue details (provided): Ubiquitous. High expression in liver, spleen, pancreas and kidney.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
