| Field | Specification |
|---|---|
| Target | |
| Alternative names | Histone deacetylase 9;HD9;3.5.1.98;Histone deacetylase 7B;HD7;HD7b;Histone deacetylase-related protein;MEF2-interacting transcription repressor MITR;HDAC9;HDAC7, HDAC7B, HDRP, KIAA0744, MITR; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human HDAC9 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone HEF-8 is a rabbit monoclonal antibody that recognizes histone deacetylase 9 (gene HDAC9) in human and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 111.3 kDa, and the supplier reports an observed band at about 160 kDa.
Histone deacetylase 9 (HDAC9) is a class IIa deacetylase that represses MEF2-dependent transcription. A short splice form known as MITR lacks the deacetylase domain but still acts as a repressor by recruiting other corepressors. HDAC9 is studied in muscle and heart gene regulation, regulatory T-cell function and vascular disease.
| Target | Histone deacetylase 9 (gene HDAC9; UniProt Q9UKV0, human) |
|---|---|
| Clone | HEF-8 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 111.3 kDa |
| Observed MW | 160 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:5000–1:20000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): K562 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): human brain tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Samples with confirmed staining (ICC/IF): HeLa cells.
Immunogen
Synthetic peptide based on the sequence of human HDAC9.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places histone deacetylase 9 in the nucleus. Tissue expression noted by UniProt (human): Broadly expressed, with highest levels in brain, heart, muscle and testis. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
