| Field | Specification |
|---|---|
| Target | |
| Alternative names | Heme oxygenase 1;HO-1;1.14.99.3;HMOX1;HO, HO1; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Heme Oxygenase 1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AEG-8 is a rabbit monoclonal antibody that recognizes heme oxygenase 1 (gene HMOX1) in human and mouse samples and is validated for Western blot, IHC, immunoprecipitation and flow cytometry. The predicted molecular weight is 32.8 kDa, and the supplier reports an observed band at about 33 kDa.
Heme oxygenase 1 (HO-1, HMOX1, also called HSP32) breaks down heme into biliverdin, ferrous iron and carbon monoxide. It is strongly induced by oxidative stress, heme and inflammation, and its products have antioxidant and anti-inflammatory effects, so HO-1 is a widely used marker of cellular stress responses and cytoprotection.
| Target | Heme oxygenase 1 (gene HMOX1; UniProt P09601, human) |
|---|---|
| Clone | AEG-8 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 32.8 kDa |
| Observed MW | 33 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
| Flow cytometry | 1:20 |
Samples with a confirmed band (WB): human U87-MG cells, human A549 cells, human 293T cells, human HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 12% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human spleen tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide based on the sequence of human heme Oxygenase 1.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt places heme oxygenase 1 in the endoplasmic reticulum membrane. Tissue expression noted by UniProt (human): Expressed at higher levels in renal cancer tissue than in normal tissue (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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Haiwen Zhang et al. (2021) Vitamin D3 Protects Mice from Diquat-Induced Oxidative Stress through the NF-κB/Nrf2/HO-1 Signaling Pathway. Oxidative Medicine and Cellular Longevity. 10.1155/2021/6776956
Ke-xin Yan et al. (2024) Taraxasterol attenuates zearalenone-induced kidney damage in mice by modulating oxidative stress and endoplasmic reticulum stress. Ecotoxicology and Environmental Safety. 10.1016/j.ecoenv.2024.117093
Heran Wang et al. (2023) Activation of the Nrf-2 pathway by pinocembrin safeguards vertebral endplate chondrocytes against apoptosis and degeneration caused by oxidative stress. Life Sciences. 10.1016/j.lfs.2023.122162
Yuandong Shao et al. (2022) Icariin protects vertebral endplate chondrocytes against apoptosis and degeneration via activating Nrf-2/HO-1 pathway. Frontiers in Pharmacology. 10.3389/fphar.2022.937502
Shilin Zhang et al. (2026) Hydroxytyrosol Enhances the Nrf2/HO-1 Signalling Pathway to Inhibit Oxidative Stress and Apoptosis and Improve Premature Ovarian Insufficiency In Vitro and In Vivo. International Journal of Molecular Sciences. 10.3390/ijms27114845
Guihe Yang et al. (2023) Astaxanthin suppresses oxidative stress and calcification in vertebral cartilage endplate via activating Nrf-2/HO-1 signaling pathway. International Immunopharmacology. 10.1016/j.intimp.2023.110159
Zhenkai Ma et al. (2023) Nrf2 protects against cartilage endplate degeneration through inhibiting NCOA4‑mediated ferritinophagy. International Journal of Molecular Medicine. 10.3892/ijmm.2023.5339
Xingang Cui et al. (2023) PTEN inhibitor VO-OHpic protects endplate chondrocytes against apoptosis and calcification via activating Nrf-2 signaling pathway. Aging-US. 10.18632/aging.204612
Ling Yang et al. (2026) Yangjing Shugan decoction targets the Sirt1/Nrf2 antioxidant pathway and gut microbiota-metabolite axis to protect against premature ovarian failure. Frontiers in Pharmacology. 10.3389/fphar.2025.1722692
