| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Heme oxygenase 1;HO-1;1.14.99.3;HMOX1;HO, HO1; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human Heme Oxygenase 1 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-HMOX1 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone AEG-8; isotype Rabbit IgG; reactivity: Human,Mouse. Reported application contexts include WB, IHC, IP, Flow (as provided in the source record). Boster Bio Anti-Heme Oxygenase 1 HMOX1 Rabbit Monoclonal Antibody catalog # M00253. Tested in WB, IHC, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse.
Key elements and design rationale
- Target: HMOX1 (Heme oxygenase 1).
- Antibody format: Monoclonal; clone AEG-8; isotype Rabbit IgG.
- Host: Rabbit.
- Species reactivity: Human,Mouse (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
HMOX1 (protein: P2X purinoceptor 1) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Exhibits cytoprotective effects since excess of free heme sensitizes cells to undergo apoptosis. Reported cellular localization context: Microsome . Endoplasmic reticulum membrane ; Peripheral membrane protein ; Cytoplasmic side . Tissue expression notes (as provided): Expressed at higher levels in renal cancer tissue than in normal tissue (at protein level). .
Research relevance and current trends
- Research context keywords from the source record include: Alzheimer's Disease,Atherosclerosis,Cancer,Cancer Metabolism,Cardiovascular,Epigenetics and Nuclear Signaling,Heart Disease,Metabolism,Metabolism Processes,Neurodegenerative Disease,Neurology Process,Neuroscience,NFKB Pathway,Nuclear Signaling,Nuclear Signaling Pathways,Pathways and Processes,Platelets,Response To Hypoxia,Signal Transduction,Signaling Pathway,Vascular Inflammation,Vasculature.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
- Flow cytometry: quantify target-positive populations and compare shifts in marker distributions.
- Immunoprecipitation (IP): enrich target complexes for downstream immunoblot or interaction analyses.
Workflow ideas (metafield): Validate HMOX1 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect HMOX1 expression by Western blot in cell or tissue lysates, Detect HMOX1 in FFPE tissue sections by immunohistochemistry, Quantify HMOX1-positive cells by flow cytometry in single-cell suspensions, Enrich HMOX1 by immunoprecipitation from lysates for downstream analysis
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 80 kDa; calculated MW: 32819 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 80 kDa
- Cellular localization (provided): Microsome . Endoplasmic reticulum membrane ; Peripheral membrane protein ; Cytoplasmic side .
- Tissue details (provided): Expressed at higher levels in renal cancer tissue than in normal tissue (at protein level). .
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
