Anti-Hemoglobin/HBA1/HBA2 Antibody Picoband®

Overview

Anti-Hemoglobin/HBA1/HBA2 Antibody Picoband® is an antibody for HBA1/HBA2 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.

Key elements and design rationale

• Target: HBA1/HBA2 (Hemoglobin subunit alpha); UniProt: P69905; NCBI Gene: 3039/3040
• Antibody format: Rabbit, Polyclonal, Rabbit IgG
• Molecular weight: 15 kDa, calculated 15258 MW
• Applications: WB, IHC, Flow Cytometry, ELISA

Vendor description (summary): Boster Bio Anti-Hemoglobin/HBA1/HBA2 Antibody Picoband® catalog # A00233-2.

Biological background

Biological context: Involved in oxygen transport from the lung to the various peripheral tissues.

Expression and localization notes: cellular localization: Secreted., tissue context: Red blood cells..

Common research applications

• Western blotting (WB): Compare HBA1/HBA2 levels across samples and conditions using appropriate loading and biological controls.
• Immunohistochemistry (IHC): Evaluate spatial distribution of HBA1/HBA2 in tissue sections, considering fixation and antigen retrieval effects.
• Flow cytometry: Quantify HBA1/HBA2-positive populations in single-cell suspensions with appropriate gating and controls.
• ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.

Notes for experimental interpretation

• Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
• Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.

Additional product notes (from provided fields)

• Background: The human alpha globin gene cluster located on chromosome 16 spans about 30 kb and includes seven loci: 5'- zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 - alpha-1 - theta - 3'. The alpha-2 (HBA2) and alpha-1 (HBA1) coding sequences are identical. These genes differ slightly over the 5' untranslated regions and the introns, but they differ significantly over the 3' untranslated regions. Two alpha chains plus two beta chains constitute HbA, which in normal adult life comprises about 97% of the total hemoglobin; alpha chains combine with delta chains to constitute HbA-2, which with HbF (fetal hemoglobin) makes up the remaining 3% of adult hemoglobin. Alpha thalassemias result from deletions of each of the alpha genes as well as deletions of both HBA2 and HBA1; some nondeletion alpha thalassemias have also been reported.
• Cross reactivity: No cross-reactivity with other proteins
• Cellular localization: Secreted.
• Tissue details: Red blood cells.
• Research category: Adaptive Immunity,Cancer,Cytokines,Immunology,Innate Immunity,Interleukins,Tumor Immunology

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.