Anti-Heparanase 1/Hpse Antibody Picoband®

SKU:BHA21001182
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Suppliers
Boster Bio
Boster Bio
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Overview
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Anti-Hpse antibody (Rabbit polyclonal, Rabbit IgG). Validated for Western blot. Reactivity: Mouse. Recognizes heparanase. Supplied in lyophilized form, ready for use in standard immunodetection workflows.
Target Hpse
Host Rabbit
Reactivity Mouse
Isotype Rabbit IgG
Application(s) WB
Options selector
Catalog no. Size Conjugation
A01313 100 ug/vial
Available Options

Select the variant that best fits your experiment.

  • Options:
    • 100 ug/vial / Carrier Free, Unconjugated
      Form: Lyophilized
      Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
      Applications: WB
      Application details: Western blot, 0.1-0.5μg/ml, Mouse
      Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
    • 100 ug/vial / APC, Biotin, Cy3, FITC, Fluoro488, Fluoro550, Fluoro594, Fluoro647, PE
      Form: Liquid
      Storage: At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing.; At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
      Applications: WB,IHC,ELISA; Flow Cytometry
      Application details: Western blot, 0.25-0.5μg/ml
      Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml
      ELISA, 0.1-0.5μg/ml
      Flow Cytometry, 1-3μg/1x106 cellsContents: Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
    • 100 ug/vial / HRP
      Form: Liquid
      Storage: At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing.
      Applications: WB,IHC,ELISA
      Application details: Western blot, 0.25-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml
      ELISA, 0.1-0.5μg/ml
      Contents: Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4.
  • Lead time: typically ships in ~2-3 business days; timing may vary by selected option.
  • Storage: varies by selected option; see option details under Options.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target Hpse
Alternative names Heparanase;3.2.1.166;Endo-glucoronidase;Heparanase 8 kDa subunit;Heparanase 50 kDa subunit;Hpse;Hpa;
UniProt # Q6YGZ1
Host Rabbit
Clonality
  • Polyclonal
Isotype
  • Rabbit IgG
Reactivity
  • Mouse
Applications
  • Western Blot
Immunogen E.coli-derived mouse Heparanase 1 recombinant protein (Position: K206-K457). Mouse Heparanase
Purification Immunogen affinity purified.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Cellular localization Lysosome membrane ; Peripheral membrane protein . Secreted . Nucleus . Proheparanase is secreted via vesicles of the Golgi. Interacts with cell membrane heparan sulfate proteoglycans (HSPGs). Endocytosed and accumulates in endosomes. Transferred to lysosomes where it is proteolytically cleaved to produce the active enzyme. Under certain stimuli, transferred to the cell surface. Colocalizes with SDC1 in endosomal/lysosomal vesicles. Accumulates in perinuclear lysosomal vesicles. Heparin retains proheparanase in the extracellular medium (By similarity). Associates with lipid rafts. .
Concentration Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Form Lyophilized
Storage Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Catalog no. (Mfr.) A01313
Main SKU BHA21001182
Rabbit Polyclonal · Picoband®

Product Overview

This rabbit polyclonal antibody targets heparanase (gene Hpse) in mouse samples and is validated for Western blot.

Heparanase (HPSE) is the main mammalian enzyme that cuts heparan sulfate chains on proteoglycans in the extracellular matrix and on cell surfaces. This releases bound growth factors and cytokines and remodels the matrix, so heparanase is studied in inflammation, angiogenesis, tumor invasion and metastasis.

Target Heparanase (gene Hpse; UniProt Q6YGZ1, mouse)
Host / Clonality / Isotype Rabbit / Polyclonal / Rabbit IgG
Reactivity Mouse
Purification Immunogen affinity purified
Form Lyophilized
Formulation Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3
Reconstitution 500 µg/mL after adding 0.2 mL distilled water
Storage As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles.

Validated Applications

Western blot 0.1–0.5 µg/mL (Mouse)

Immunogen

Recombinant mouse Heparanase 1 fragment (Lys206–Lys457), expressed in E. coli.

Reactivity Notes

The supplier lists reactivity with mouse. UniProt describes heparanase as secreted and also located in the lysosome membrane and nucleus. Tissue expression noted by UniProt (mouse): Expressed in skin, mainly in the stratum granulosum and the first layer of the stratum corneum in the upper part of the epidermis.

Safety

Handling: the formulation contains sodium azide as a preservative. Wear gloves, avoid contact with skin and eyes, and dispose of waste according to local regulations. For research use only; not for diagnostic or therapeutic procedures.
Q.Which applications is this antibody validated for, and at what dilution?
A.Validated applications with suggested starting dilutions: Western blot 0.1–0.5 µg/mL (Mouse). Optimal dilutions should be determined by each laboratory.
Q.Which species does this antibody react with?
A.Validated reactivity: Mouse. Other species are not listed by the supplier.
Q.What immunogen was used to raise this antibody?
A.Recombinant mouse Heparanase 1 fragment (Lys206–Lys457), expressed in E. coli.
Q.How should this antibody be stored?
A.As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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Try Celltrypse Free – Request Your Sample Today

Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

Try Celltrypse Free – Request Your Sample Today

Try Celltrypse Free – Request Your Sample Today

Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

Try Celltrypse Free – Request Your Sample Today