| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Beta-hexosaminidase subunit beta;3.2.1.52;Beta-N-acetylhexosaminidase subunit beta;Hexosaminidase subunit B;Cervical cancer proto-oncogene 7 protein;HCC-7;N-acetyl-beta-glucosaminidase subunit beta;Beta-hexosaminidase subunit beta chain B;Beta-hexosaminidase subunit beta chain A;HEXB;HCC7; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human HEXB recombinant protein (Position: K381-M556). Human HEXB shares 75% and 73% amino acid (aa) sequences identity with mouse and rat HEXB, respectively. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of HEXB (Beta-hexosaminidase subunit beta) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-HEXB Antibody Picoband® catalog # PB9211. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E.coli-derived human HEXB recombinant protein (Position: K381-M556). Human HEXB shares 75% and 73% amino acid (aa) sequences identity with mouse and rat HEXB, respectively. (reported region: K381-M556).
- Molecular weight context: reported MW: 63 kDa; calculated MW: 63111 MW
- Reactivity: Human
- Applications: IHC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Beta-hexosaminidase subunit beta; Beta-hexosaminidase subunit beta. Beta-hexosaminidase subunit beta (HEXB) is an enzyme that in humans is encoded by the HEXB gene. It is mapped to 5q13.3. HEXB is the beta subunit of the lysosomal enzyme beta-hexosaminidase that, together with the cofactor GM2 activator protein, catalyzes the degradation of the ganglioside GM2, and other molecules containing terminal N-acetyl hexosamines. Beta subunit gene mutations lead to Sandhoff disease (GM2-gangliosidosis type II). It has been found that HEXB is a peptidoglycan hydrolase and it is involved in restricting mycobacteria growth even before the onset of adaptive immunity. Functional note: Responsible for the degradation of GM2 gangliosides, and a variety of other molecules containing terminal N-acetyl hexosamines, in the brain and other tissues. Reported localization: Lysosome. Expression/tissue context: Detected in brain, in particular in amygdala and hippocampus, while expression in caudate nucleus, corpus callosum, substantia nigra, subthalamic nucleus and thalamus is very low or not detectable. Detected at very low levels in muscle and pancreas. .
Research relevance and current trends
- Energy Metabolism: Researchers commonly examine how HEXB (Beta-hexosaminidase subunit beta) relates to this theme using model systems and orthogonal readouts.
- Energy Transfer Pathways: Researchers commonly examine how HEXB (Beta-hexosaminidase subunit beta) relates to this theme using model systems and orthogonal readouts.
- Metabolic Signaling Pathways: Researchers commonly examine how HEXB (Beta-hexosaminidase subunit beta) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative HEXB (Beta-hexosaminidase subunit beta) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of HEXB (Beta-hexosaminidase subunit beta) across tissue regions and cell types using matched controls.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Family / similarity context: Belongs to the glycosyl hydrolase 20 family.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
