| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Hexokinase-1; Brain form hexokinase; Hexokinase type I; HK I; HK1 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human Hexokinase 1/HK1 recombinant protein (Position: D17-R323). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-Hexokinase 1/HK1 Antibody Picoband® (monoclonal, 2I4) is an antibody for HK1 detection raised in Mouse (Monoclonal, clone Clone: 2I4, Mouse IgG2b), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, IHC-F, IF, ICC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: HK1 (hexokinase 1); UniProt: P19367
- Antibody format: Mouse, Monoclonal, clone Clone: 2I4, Mouse IgG2b
- Molecular weight: 120 kDa
- Applications: WB, IHC, IHC-F, IF, ICC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-Hexokinase 1/HK1 Antibody Picoband® (monoclonal, 2I4) catalog # M01504-1.
Biological background
Biological context: Catalyzes the phosphorylation of various hexoses, such as D-glucose, D-glucosamine, D-fructose, D-mannose and 2-deoxy-D-glucose, to hexose 6-phosphate (D-glucose 6-phosphate, D-glucosamine 6-phosphate, D-fructose 6-phosphate, D-mannose 6-phosphate and 2-deoxy-D-glucose 6-phosphate, respectively). Does not phosphorylate N-acetyl-D-glucosamine. Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate. Involved in innate immunity and inflammation by acting as a pattern recognition receptor for bacterial peptidoglycan. When released in the cytosol, N-acetyl-D-glucosamine component of bacterial peptidoglycan inhibits the hexokinase activity of HK1 and causes its dissociation from mitochondrial outer membrane, thereby activating the NLRP3 inflammasome.
Expression and localization notes: cellular localization: Cytosol. Mitochondrion outer membrane. Peripheral membrane protein., tissue context: Ubiquitous..
Common research applications
- Western blotting (WB): Compare HK1 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of HK1 in tissue sections, considering fixation and antigen retrieval effects.
- Immunofluorescence / ICC: Assess subcellular localization patterns and co-localization with compartment markers in cultured cells.
- Flow cytometry: Quantify HK1-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Specificity: No cross reactivity with other proteins.
- Background: Hexokinase-1 (HK1) is an enzyme that in humans is encoded by the HK1 gene on chromosome 10. It is mapped to 10q22.1. Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in several transcript variants which encode different isoforms, some of which are tissue-specific.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Cytosol. Mitochondrion outer membrane. Peripheral membrane protein.
- Tissue details: Ubiquitous.
- Research category: DNA/RNA,Epigenetics and Nuclear Signaling,RNA Processing
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
