Anti-HIC5/TGFB1I1 Antibody Picoband®

Overview

Anti-HIC5/TGFB1I1 Antibody Picoband® is an antibody for TGFB1I1 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Monkey. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.

Key elements and design rationale

• Target: TGFB1I1 (solute carrier family 12 member 5); UniProt: O43294
• Antibody format: Rabbit, Polyclonal, Rabbit IgG
• Molecular weight: 75 kDa
• Applications: WB, IHC, Flow Cytometry, ELISA

Vendor description (summary): Boster Bio Anti-HIC5/TGFB1I1 Antibody Picoband® catalog # A04630-2.

Biological background

Biological context: Mediates electroneutral potassium-chloride cotransport in mature neurons and is required for neuronal Cl- homeostasis. As major extruder of intracellular chloride, it establishes the low neuronal Cl- levels required for chloride influx after binding of GABA-A and glycine to their receptors, with subsequent hyperpolarization and neuronal inhibition. Involved in the regulation of dendritic spine formation and maturation.

Expression and localization notes: cellular localization: Membrane. Multi-pass membrane protein., tissue context: Brain specific. Detected in neuronal cells..

Common research applications

• Western blotting (WB): Compare TGFB1I1 levels across samples and conditions using appropriate loading and biological controls.
• Immunohistochemistry (IHC): Evaluate spatial distribution of TGFB1I1 in tissue sections, considering fixation and antigen retrieval effects.
• Flow cytometry: Quantify TGFB1I1-positive populations in single-cell suspensions with appropriate gating and controls.
• ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.

Notes for experimental interpretation

• Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
• Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.

Additional product notes (from provided fields)

• Background: Transforming growth factor beta-1-induced transcript 1 protein is a protein that in humans is encoded by the TGFB1I1 gene. This gene encodes a coactivator of the androgen receptor, a transcription factor which is activated by androgen and has a key role in male sexual differentiation. The encoded protein is thought to regulate androgen receptor activity and may have a role to play in the treatment of prostate cancer. Multiple transcript variants encoding different isoforms have been found for this gene.
• Cross reactivity: No cross-reactivity with other proteins.
• Cellular localization: Membrane. Multi-pass membrane protein.
• Tissue details: Brain specific. Detected in neuronal cells.
• Research category: Neuroscience,Neurotransmission,Potassium Channels,Receptors / Channels

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.