| Field | Specification |
|---|---|
| Target | |
| Alternative names | Hypoxia-inducible factor 1-alpha;HIF-1-alpha;HIF1-alpha;ARNT-interacting protein;Basic-helix-loop-helix-PAS protein MOP1;Class E basic helix-loop-helix protein 78;bHLHe78;Member of PAS protein 1;PAS domain-containing protein 8;HIF1A;BHLHE78, MOP1, PASD8; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminal of human HIF-1-alpha, different from the related mouse and rat sequences by three amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets hypoxia-inducible factor 1-alpha (gene HIF1A) in human, mouse and rat samples and is validated for IHC and Western blot. The predicted molecular weight is 92.7 kDa, and the supplier reports an observed band at about 120 kDa.
HIF-1α (HIF1A) is the oxygen-regulated subunit of hypoxia-inducible factor 1. In normal oxygen it is hydroxylated and rapidly degraded, but under hypoxia it accumulates, pairs with ARNT (HIF-1β) and switches on genes for erythropoietin, VEGF, glucose uptake and glycolysis. It is a central marker in studies of hypoxia, angiogenesis, tumor metabolism and ischemia.
| Target | Hypoxia-inducible factor 1-alpha (gene HIF1A; UniProt Q16665, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 92.7 kDa |
| Observed MW | 120 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Mouse, Rat) |
|---|---|
| Western blot | 0.1–0.5 µg/mL (Human, Mouse) |
Samples with a confirmed band (WB): human HepG2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 8% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse intestine tissue, rat intestine tissue, human intestinal cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Immunogen
Synthetic peptide based on the sequence of at the C-terminal of human HIF-1-alpha. It differs from the mouse and rat sequences at three residues.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places hypoxia-inducible factor 1-alpha in the cytoplasm, nucleus and nucleus speckle. Tissue expression noted by UniProt (human): Expressed in most tissues with highest levels in kidney and heart. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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