| Field | Specification |
|---|---|
| Target | |
| Alternative names | Endothelial PAS domain-containing protein 1;EPAS-1;Hypoxia-inducible factor 2-alpha;HIF-2-alpha;HIF2-alpha;Epas1;Hif2a; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human HIF-2-alpha, identical to the related mouse and rat sequence. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets endothelial PAS domain-containing protein 1 (gene Epas1) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 96.7 kDa, and the supplier reports an observed band at about 120 kDa.
HIF-2α (EPAS1) is an oxygen-regulated transcription factor. Under low oxygen it escapes VHL-mediated degradation, pairs with ARNT (HIF-1β) and activates hypoxia-responsive genes such as VEGF and erythropoietin. HIF-2α is studied in vascular biology, erythropoiesis, high-altitude adaptation and clear cell renal carcinoma.
| Target | Endothelial PAS domain-containing protein 1 (gene Epas1; UniProt Q9JHS1, rat) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg thimerosal; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 96.7 kDa |
| Observed MW | 120 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|
Samples with a confirmed band (WB): rat thymus tissue, rat testis tissue, human MCF-7 cells, human HeLa cells, human Jurkat cells, mouse thymus tissue, mouse lun tissue, rat brain tissue, rat RH35 cells, rat small intestine tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Immunogen
Synthetic peptide from the middle region of human HIF-2-alpha. The peptide sequence is conserved in mouse and rat.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places endothelial PAS domain-containing protein 1 in the nucleus and nucleus speckle. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Emel Baloglu (2023) HIF-2α Controls Expression and Intracellular Trafficking of the α2-Subunit of Na,K-ATPase in Hypoxic H9c2 Cardiomyocytes. Biomedicines. 10.3390/biomedicines11112879
