| Field | Specification |
|---|---|
| Target | |
| Alternative names | Endothelial PAS domain-containing protein 1;EPAS-1;Basic-helix-loop-helix-PAS protein MOP2;Class E basic helix-loop-helix protein 73;bHLHe73;HIF-1-alpha-like factor;HLF;Hypoxia-inducible factor 2-alpha;HIF-2-alpha;HIF2-alpha;Member of PAS protein 2;PAS domain-containing protein 2;EPAS1;BHLHE73, HIF2A, MOP2, PASD2; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human HIF-2 alpha |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ADD-5 is a rabbit monoclonal antibody that recognizes endothelial PAS domain-containing protein 1 (gene EPAS1) in human, mouse and rat samples and is validated for Western blot, ICC/IF and flow cytometry. The predicted molecular weight is 96.5 kDa, and the supplier reports an observed band at about 96–120 kDa.
HIF-2α (EPAS1) is an oxygen-regulated transcription factor. Under low oxygen it escapes VHL-mediated degradation, pairs with ARNT (HIF-1β) and activates hypoxia-responsive genes such as VEGF and erythropoietin. HIF-2α is studied in vascular biology, erythropoiesis, high-altitude adaptation and clear cell renal carcinoma.
| Target | Endothelial PAS domain-containing protein 1 (gene EPAS1; UniProt Q99814, human) |
|---|---|
| Clone | ADD-5 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 96.5 kDa |
| Observed MW | 96–120 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:20 |
Immunogen
Synthetic peptide based on the sequence of human HIF-2 alpha.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places endothelial PAS domain-containing protein 1 in the nucleus and nucleus speckle. Tissue expression noted by UniProt (human): Expressed in most tissues, with highest levels in placenta, lung and heart.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Hu Qiang et al. (2026) Divergent mechanisms, unified therapy: saracatinib targets Src/HIF signaling in endothelial and microglial cells to Treat retinopathy. Biochemical Pharmacology. 10.1016/j.bcp.2026.118028
Xingzhi Jing et al. (2024) HIF-2α/TFR1 mediated iron homeostasis disruption aggravates cartilage endplate degeneration through ferroptotic damage and mtDNA release: A new mechanism of intervertebral disc degeneration. Journal of Orthopaedic Translation. 10.1016/j.jot.2024.03.005
Li Ma et al. (2014) 2-Methoxyestradiol synergizes with sorafenib to suppress hepatocellular carcinoma by simultaneously dysregulating hypoxia-inducible factor-1 and -2. Cancer Letters. 10.1016/j.canlet.2014.09.011
Xue-Pu Sun et al. (2013) Up-regulation of survivin by AKT and hypoxia-inducible factor 1α contributes to cisplatin resistance in gastric cancer. FEBS Journal. 10.1111/febs.12577
Changjun He et al. (2011) Downregulating hypoxia-inducible factor-2α improves the efficacy of doxorubicin in the treatment of hepatocellular carcinoma. Cancer Science. 10.1111/j.1349-7006.2011.02177.x
Jianyi Cui et al. (2017) Gambogic acid ameliorates diabetes-induced proliferative retinopathy through inhibition of the HIF-1α/VEGF expression via targeting PI3K/AKT pathway. Life Sciences. 10.1016/j.lfs.2017.11.007
Yang Sheng-Li et al. (2013) The Correlation of Expression Levels of HIF-1α and HIF-2α in Hepatocellular Carcinoma with Capsular Invasion, Portal Vein Tumor Thrombi and Patients' Clinical Outcome. Japanese Journal of Clinical Oncology. 10.1093/jjco/hyt194
