Anti-HIF-2 alpha EPAS1 Rabbit Monoclonal Antibody

Overview

This product is an anti-EPAS1 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone ADD-5; isotype Rabbit IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, ICC, IF, Flow (as provided in the source record). Boster Bio Anti-HIF-2 alpha EPAS1 Rabbit Monoclonal Antibody catalog # M01248. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Key elements and design rationale

• Target: EPAS1 (Endothelial PAS domain-containing protein 1).
• Antibody format: Monoclonal; clone ADD-5; isotype Rabbit IgG.
• Host: Rabbit.
• Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).

This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.

Biological background

EPAS1 (protein: Glycogen synthase kinase-3 beta (gsk3b)) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation seems to require recruitment of transcriptional coactivators such as CREBPB and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD. Reported cellular localization context: Nucleus . Nucleus speckle . Colocalizes with HIF3A in the nucleus and speckles. . Tissue expression notes (as provided): Expressed in most tissues, with highest levels in placenta, lung and heart. Selectively expressed in endothelial cells.

Research relevance and current trends

• Research context keywords from the source record include: Cancer,Cancer Metabolism,Cardiovascular,2339,Domain Families,Epigenetics and Nuclear Signaling,Hlh/Leucine Zipper,Hypoxia,Invasion/Microenvironment,Metabolic Signaling Pathways,Metabolism,Metabolism Processes,Nucleotide Metabolism,Pathways and Processes,Response To Hypoxia,Transcription.
• Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
• Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.

Common research applications

• Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
• Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
• Flow cytometry: quantify target-positive populations and compare shifts in marker distributions.

Workflow ideas (metafield): Validate EPAS1 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect EPAS1 expression by Western blot in cell or tissue lysates, Localize EPAS1 by immunofluorescence/immunocytochemistry in cultured cells, Quantify EPAS1-positive cells by flow cytometry in single-cell suspensions

Notes for experimental interpretation

• Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
• Apparent molecular weight may vary by sample type and processing (observed MW: 80 kDa; calculated MW: 96459 MW).
• Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.

Additional product details (from the source record)

• Molecular weight (observed): 80 kDa
• Cellular localization (provided): Nucleus . Nucleus speckle . Colocalizes with HIF3A in the nucleus and speckles. .
• Tissue details (provided): Expressed in most tissues, with highest levels in placenta, lung and heart. Selectively expressed in endothelial cells.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.