| Field | Specification |
|---|---|
| Target | |
| Alternative names | Hypoxia-inducible factor 3-alpha ;HIF-3-alpha ;HIF3-alpha;Basic-helix-loop-helix-PAS protein MOP7;Class E basic helix-loop-helix protein 17;bHLHe17;HIF3-alpha-1;Inhibitory PAS domain protein;IPAS;Member of PAS protein 7;PAS domain-containing protein 7;HIF3A ;BHLHE17, MOP7, PASD7; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human HIF3, different from the related rat and mouse sequences by five amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets hypoxia-inducible factor 3-alpha (gene HIF3A) in rat and human samples and is validated for Western blot. The predicted molecular weight is 72.4 kDa, and the supplier reports an observed band at about 49 kDa.
HIF-3α (HIF3A) is the third oxygen-regulated α subunit of the hypoxia-inducible factor family. It can partner with ARNT, and several of its splice forms act as dominant-negative regulators that reduce HIF-1α and HIF-2α target gene activation, so it is studied as a modulator of the hypoxic response.
| Target | Hypoxia-inducible factor 3-alpha (gene HIF3A; UniProt Q9Y2N7, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Rat, Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg thimerosal; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 72.4 kDa |
| Observed MW | 49 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Rat, Human) |
|---|
Samples with a confirmed band (WB): rat brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide from the C-terminal region of human HIF3. It differs from the rat and mouse sequences at five residues.
Reactivity Notes
The supplier lists reactivity with rat and human. UniProt places hypoxia-inducible factor 3-alpha in the nucleus, cytoplasm, nucleus speckle and mitochondrion. Tissue expression noted by UniProt (human): Expressed in vascular cells (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
