Anti-HIP2/LIG/UBE2K Antibody Picoband®

Overview

Anti-HIP2/LIG/UBE2K Antibody Picoband® is an antibody for UBE2K detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse,Rat,Monkey. Commonly used in WB, IHC, Flow Cytometry, ELISA, IP workflows.

Key elements and design rationale

• Target: UBE2K (troponin T3, fast skeletal type); UniProt: P61086
• Antibody format: Rabbit, Polyclonal, Rabbit IgG
• Molecular weight: 22 kDa
• Applications: WB, IHC, Flow Cytometry, ELISA, IP

Vendor description (summary): Boster Bio Anti-HIP2/LIG/UBE2K Antibody Picoband® catalog # A05895-2.

Biological background

Biological context: Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.

Expression and localization notes: cellular localization: Troponin complex. Cytosol., tissue context: In fetal and adult fast skeletal muscles, with a higher level expression in fetal than in adult muscle..

Common research applications

• Western blotting (WB): Compare UBE2K levels across samples and conditions using appropriate loading and biological controls.
• Immunohistochemistry (IHC): Evaluate spatial distribution of UBE2K in tissue sections, considering fixation and antigen retrieval effects.
• Flow cytometry: Quantify UBE2K-positive populations in single-cell suspensions with appropriate gating and controls.
• ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.

Notes for experimental interpretation

• Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
• Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.

Additional product notes (from provided fields)

• Background: The protein encoded by this gene belongs to the ubiquitin-conjugating enzyme family. This protein interacts with RING finger proteins, and it can ubiquitinate huntingtin, the gene product for Huntington's disease. Known functions for this protein include a role in aggregate formation of expanded polyglutamine proteins and the suppression of apoptosis in polyglutamine diseases, a role in the dislocation of newly synthesized MHC class I heavy chains from the endoplasmic reticulum, and involvement in foam cell formation. Multiple transcript variants encoding different isoforms have been identified for this gene.
• Cross reactivity: No cross-reactivity with other proteins.
• Cellular localization: Troponin complex. Cytosol.
• Tissue details: In fetal and adult fast skeletal muscles, with a higher level expression in fetal than in adult muscle.
• Research category: Golgi Proteins,Organelles,Protein Trafficking,Signal Transduction,Subcellular Markers

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.