| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Cell division control protein 45 homolog;PORC-PI-1;CDC45;CDC45L, CDC45L2;UNQ374/PRO710; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Gene ID | |
| Host | |
| Immunogen | A synthesized peptide derived from human HIPK2 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-HIPK2 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone 21H33; isotype IgG; reactivity: Human,Mouse. Reported application contexts include WB, ICC, IF, IP, Flow (as provided in the source record). Boster Bio Anti-HIPK2 Rabbit Monoclonal Antibody catalog # M01371. Tested in WB, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse.
Key elements and design rationale
- Target: HIPK2 (Cell division control protein 45 homolog).
- Antibody format: Monoclonal; clone 21H33; isotype IgG.
- Host: Rabbit.
- Species reactivity: Human,Mouse (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
HIPK2 (protein: Glycogen synthase kinase-3 beta (gsk3b)) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Required for initiation of chromosomal DNA replication. Reported cellular localization context: Cytoplasm. Nucleus. Tissue expression notes (as provided): Widely expressed, highest levels are found in adult testis and thymus and in fetal liver.
Research relevance and current trends
- Research context keywords from the source record include: Cell Biology,Cell Cycle,Cell Division,DNA/RNA,DNA Synthesis,Epigenetics and Nuclear Signaling.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and compare shifts in marker distributions.
- Immunoprecipitation (IP): enrich target complexes for downstream immunoblot or interaction analyses.
Workflow ideas (metafield): Validate HIPK2 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect HIPK2 expression by Western blot in cell or tissue lysates, Localize HIPK2 by immunofluorescence/immunocytochemistry in cultured cells, Quantify HIPK2-positive cells by flow cytometry in single-cell suspensions, Enrich HIPK2 by immunoprecipitation from lysates for downstream analysis
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 130 kDa, 100 kDa; calculated MW: 65569 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 130 kDa, 100 kDa
- Cellular localization (provided): Cytoplasm. Nucleus.
- Tissue details (provided): Widely expressed, highest levels are found in adult testis and thymus and in fetal liver.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
