Anti-His Tag Antibody (Mouse Monoclonal)

What it means: Primary antibody — directly recognizes and binds the target antigen in your sample.

Why it matters:

• First layer in indirect detection: must be paired with a compatible secondary antibody
• Antigen specificity is determined by the primary antibody's epitope selection
• Clonality determines batch consistency (monoclonal) vs. signal breadth (polyclonal)

How to interpret here: As a primary antibody, confirm secondary antibody compatibility (host species and isotype) before setting up your detection system.

What it means: Host species is Mouse — the animal in which this antibody was raised and produced.

Why it matters:

• Secondary antibody must be anti-Mouse — verify species compatibility with your detection kit
• Do not pair with another primary antibody from Mouse in multiplex staining
• Mouse antibodies follow well-established secondary antibody pairing protocols

How to interpret here: Select an anti-Mouse secondary antibody for indirect detection. For direct conjugate options, check the variant selector.

What it means: Species reactivity of Other — the species whose target protein has been validated to react with this antibody.

Why it matters:

• Only use with samples from validated species — unlisted species are not guaranteed
• Cross-reactivity depends on target protein sequence conservation across species
• Confirm species match before ordering when working with non-standard animal models

How to interpret here: This antibody is validated for Other samples. For species not listed, check protein sequence homology and consider independent validation.

What it means: Unconjugated — no fluorophore, enzyme, or biotin label. A secondary antibody or detection reagent is required.

Why it matters:

• Maximum flexibility: pair with any compatible secondary antibody or detection system
• Signal amplification possible by layering HRP-polymer or other detection reagents
• Suitable for custom detection and multicolor panel design

How to interpret here: As an unconjugated primary, you need a compatible secondary antibody. Verify host species and isotype of your secondary.

What it means: Isotype IgG2 — the antibody heavy-chain class, which determines secondary antibody requirements.

Why it matters:

• Secondary antibody or detection kit must be specific for IgG2
• Select the matching isotype control for proper background correction in flow cytometry
• IgG subclasses differ in Fc receptor binding, complement activation, and effector function

How to interpret here: Ensure your secondary antibody is specific for IgG2. Use a matched isotype control to assess non-specific background in your assay.

What it means: Validated for WB, IHC, IP, ICC/IF — confirmed by the manufacturer.

Why it matters:

• Validated applications reflect tested and confirmed conditions for reliable detection
• Non-listed applications require independent validation before routine use
• Each application may need different sample prep, dilution, and detection systems

How to interpret here: Refer to the datasheet for recommended dilution ranges and positive control tissues. For non-listed applications, perform a titration first.