| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Neuromodulin; Axonal membrane protein GAP-43; Growth-associated protein 43; Neural phosphoprotein B-50; pp46; GAP43 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human Histidine decarboxylase/HDC recombinant protein (Position: R12-V662). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-Histidine decarboxylase/HDC Antibody Picoband® is an antibody for HDC detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: HDC (growth associated protein 43); UniProt: P19113
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 74 kDa, calculated 50433 MW
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-Histidine decarboxylase/HDC Antibody Picoband® catalog # A01876-1.
Biological background
Biological context: This protein is associated with nerve growth. It is a major component of the motile 'growth cones' that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction.
Expression and localization notes: cellular localization: Cell membrane. Peripheral membrane protein. Cytoplasmic side. Growth cone membrane. Filopodium membrane. Synapse. Perikaryon. Dendrite. Axon. Cytoplasm., tissue context: Isoform 1 and isoform 2 are expressed in liver, colon and small intestine. Isoform 2 but not isoform 1 is expressed in kidney. Isoform 1 and isoform 2 are not expressed in esophagus. Not expressed in skin..
Common research applications
- Western blotting (WB): Compare HDC levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of HDC in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify HDC-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: HDC(Histidine decarboxylase) is the enzyme that catalyzes the reaction that produces histamine from histidine with the help of vitamin B6. The HDC gene is mapped on 15q21.2. The deduced 662-amino acid protein has a molecular mass of 74,148 Da. The gene contains 12 exons spanning approximately 24 kb. The biogenic amine histamine is an important modulator of numerous physiologic processes, including neurotransmission, gastric acid secretion, and smooth muscle tone. The biosynthesis of histamine from histidine is catalyzed by the enzyme L-histidine decarboxylase. This homodimeric enzyme is a pyridoxal phosphate(PLP)-dependent decarboxylase and is highly specific for its histidine substrate. Ercan-Sencicek et al. noted that animal studies had shown that lack of Hdc in mice results in increased locomotor and stereotypic behaviors, as well as increased anxiety.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Cell membrane. Peripheral membrane protein. Cytoplasmic side. Growth cone membrane. Filopodium membrane. Synapse. Perikaryon. Dendrite. Axon. Cytoplasm.
- Tissue details: Isoform 1 and isoform 2 are expressed in liver, colon and small intestine. Isoform 2 but not isoform 1 is expressed in kidney. Isoform 1 and isoform 2 are not expressed in esophagus. Not expressed in skin.
- Research category: Drug Metabolism,Metabolic Signaling Pathways,Metabolism,Pathways and Processes,Signal Transduction
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
