| Field | Specification |
|---|---|
| Target | |
| Alternative names | Histone H3.3;H3F3A;H3.3A, H3F3;PP781;H3F3B;H3.3B; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Histone H3.3 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone EBF-8 is a rabbit monoclonal antibody that recognizes histone H3.3 (gene H3-3A) in human, mouse and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 15.3 kDa, and the supplier reports an observed band at about 15 kDa.
Histone H3.3 (H3-3A) is a replacement H3 variant deposited into chromatin independently of DNA replication, mainly at actively transcribed genes, enhancers and telomeres. It is the dominant H3 in non-dividing cells. Recurrent H3.3 mutations such as K27M and G34R/V are established drivers of pediatric gliomas, making H3.3 a major focus of epigenetics and cancer research.
| Target | Histone H3.3 (gene H3-3A; UniProt P84243, human) |
|---|---|
| Clone | EBF-8 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 15.3 kDa |
| Observed MW | 15 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with confirmed staining (IHC): mouse stomach tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places histone H3.3 in the nucleus and chromosome. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Meena Kusi et al. (2022) 2-Hydroxyglutarate destabilizes chromatin regulatory landscape and lineage fidelity to promote cellular heterogeneity. Cell Reports. 10.1016/j.celrep.2021.110220
Tian-Cong Liu et al. (2024) METTL14-mediated upregulation of lncRNA HOTAIR represses PP1α expression by promoting H3K4me1 demethylation in oxycodone-treated mice. CNS Neuroscience & Therapeutics. 10.1111/cns.14830
Shenghui Hu et al. (2025) SIRT1-mediated deacetylation and activation of MEK/ERK pathway decreased IL-6 in spinal dorsal horn to promote oxycodone tolerance. Neuropharmacology. 10.1016/j.neuropharm.2025.110585
Liu Hua et al. (2025) Getah virus nonstructural protein 2 suppresses interferon-beta production by interrupting interferon regulatory factor 3 activation. Veterinary Research. 10.1186/s13567-025-01547-3
Xuefeng Yang et al. (2023) Porcine circovirus type 2 infection inhibits macrophage M1 polarization induced by other pathogens via viral capsid protein and host gC1qR protein. Veterinary Microbiology. 10.1016/j.vetmic.2023.109871
Xingchen Wu et al. (2021) Porcine circovirus type 2 infection attenuates the K63-linked ubiquitination of STING to inhibit IFN-β induction via p38-MAPK pathway. Veterinary Microbiology. 10.1016/j.vetmic.2021.109098
Zhenyu Wang et al. (2022) Porcine circovirus type 2 infection inhibits the activation of type I interferon signaling via capsid protein and host gC1qR. Veterinary Microbiology. 10.1016/j.vetmic.2022.109354
Haixin Liu et al. (2024) IL-10 upregulates SOCS3 to inhibit type I interferon signaling to promote PoRVA replication in intestinal epithelial cells. Veterinary Microbiology. 10.1016/j.vetmic.2024.110259
