Anti-Histone H3 (di methyl K9) HIST1H3A Rabbit Monoclonal Antibody

Overview

This product is an anti-HIST1H3A antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone GBI-8; isotype Rabbit IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, ICC, IF (as provided in the source record). Boster Bio Anti-Histone H3 (di methyl K9) HIST1H3A Rabbit Monoclonal Antibody catalog # M12477-2. Tested in WB, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.

Key elements and design rationale

• Target: HIST1H3A (Histone H3.1).
• Antibody format: Monoclonal; clone GBI-8; isotype Rabbit IgG.
• Host: Rabbit.
• Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).

This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.

Biological background

HIST1H3A (protein: T-cell surface glycoprotein CD3 zeta chain) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Reported cellular localization context: Nucleus. Chromosome. Tissue expression notes (as provided): Highly expressed in the brain, with higher expression during development than in adult. Expressed also in mammary glands, testis, colon and kidney. .

Research relevance and current trends

• Research context keywords from the source record include: Epigenetics and Nuclear Signaling,Histones,Unmodified.
• Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
• Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.

Common research applications

• Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
• Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.

Workflow ideas (metafield): Validate HIST1H3A antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect HIST1H3A expression by Western blot in cell or tissue lysates, Localize HIST1H3A by immunofluorescence/immunocytochemistry in cultured cells

Notes for experimental interpretation

• Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
• Apparent molecular weight may vary by sample type and processing (observed MW: 15 kDa; calculated MW: 15404 MW).
• Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.

Additional product details (from the source record)

• Molecular weight (observed): 15 kDa
• Cellular localization (provided): Nucleus. Chromosome.
• Tissue details (provided): Highly expressed in the brain, with higher expression during development than in adult. Expressed also in mammary glands, testis, colon and kidney. .

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.