| Field | Specification |
|---|---|
| Target | |
| Alternative names | HLA class II histocompatibility antigen, DRB1 beta chain; Human leukocyte antigen DRB1; HLA-DRB1 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human HLA-Drb1. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AFGA-8 is a rabbit monoclonal antibody that recognizes HLA class II histocompatibility antigen, DRB1 beta chain (gene HLA-DRB1) in human samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 30.0 kDa, and the supplier reports an observed band at about 30 kDa.
HLA-DRB1 encodes the main β chain of HLA-DR, which pairs with HLA-DRA on antigen-presenting cells to show peptides to CD4+ helper T cells. It is the most polymorphic class II gene and forms most of the peptide-binding groove, so DRB1 alleles are central to transplant matching and autoimmune disease genetics.
| Target | HLA class II histocompatibility antigen, DRB1 beta chain (gene HLA-DRB1; UniProt P01911, human) |
|---|---|
| Clone | AFGA-8 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 30.0 kDa |
| Observed MW | 30 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): Ramos cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human HLA-Drb1.
Reactivity Notes
The supplier lists reactivity with human. UniProt places HLA class II histocompatibility antigen, DRB1 beta chain in the cell membrane, endoplasmic reticulum membrane, lysosome membrane and late endosome membrane. Tissue expression noted by UniProt (human): Expressed in professional APCs: monocyte/macrophages, dendritic cells and B cells (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
