| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Helicase-like transcription factor;3.6.4.-;6.3.2.-;DNA-binding protein/plasminogen activator inhibitor 1 regulator;HIP116;RING finger protein 80;SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 3;Sucrose nonfermenting protein 2-like 3;HLTF;HIP116A, RNF80, SMARCA3, SNF2L3, ZBU1; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E. coli-derived human HLTF recombinant protein (Position: S911-L1009). Human HLTF shares 92.9% amino acid (aa) sequence identity with mouse HLTF. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of HLTF (Helicase-like transcription factor) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-HLTF Antibody Picoband® catalog # PB10070. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E. coli-derived human HLTF recombinant protein (Position: S911-L1009). Human HLTF shares 92.9% amino acid (aa) sequence identity with mouse HLTF. (reported region: S911-L1009).
- Molecular weight context: reported MW: 114 kDa; calculated MW: 113929 MW
- Reactivity: Human,Mouse,Rat
- Applications: Flow Cytometry, IF, ICC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Helicase-like transcription factor; Helicase-like transcription factor. Helicase-like transcription factor is an enzyme that in humans is encoded by the HLTF gene. This gene encodes a member of the SWI/SNF family. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein contains a RING finger DNA binding motif. Two transcript variants encoding the same protein have been found for this gene. However, use of an alternative translation start site produces an isoform that is truncated at the N-terminus compared to the full-length protein. Functional note: Has both helicase and E3 ubiquitin ligase activities. Possesses intrinsic ATP-dependent nucleosome-remodeling activity; This activity may be required for transcriptional activation or repression of specific target promoters (By similarity). These may include the SERPINE1 and HIV-1 promoters and the SV40 enhancer, to which this protein can bind ly. Plays a role in error-free postreplication repair (PRR) of damaged DNA and maintains genomic stability through acting as a ubiquitin ligase for 'Lys-63'-linked polyubiquitination of chromatin-bound PCNA. . Reported localization: Cytoplasm . Nucleus . Nucleus, nucleolus . Nucleus, nucleoplasm . Nuclear localization is stimulated by progesterone. . Expression/tissue context: Expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle. .
Research relevance and current trends
- Cancer: Researchers commonly examine how HLTF (Helicase-like transcription factor) relates to this theme using model systems and orthogonal readouts.
- Cancer Susceptibility: Researchers commonly examine how HLTF (Helicase-like transcription factor) relates to this theme using model systems and orthogonal readouts.
- 2339: Researchers commonly examine how HLTF (Helicase-like transcription factor) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative HLTF (Helicase-like transcription factor) levels across conditions; band patterns may reflect isoforms and processing.
- IF/ICC: evaluate subcellular localization and co-localization patterns; signal can depend on fixation/permeabilization and epitope accessibility.
- Flow cytometry: quantify target-positive populations and shifts in expression; gating strategy and background staining controls are essential.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
