| Field | Specification |
|---|---|
| Target | |
| Alternative names | Helicase-like transcription factor;3.6.4.-;6.3.2.-;DNA-binding protein/plasminogen activator inhibitor 1 regulator;HIP116;RING finger protein 80;SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 3;Sucrose nonfermenting protein 2-like 3;HLTF;HIP116A, RNF80, SMARCA3, SNF2L3, ZBU1; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human HLTF recombinant protein (Position: S911-L1009). Human HLTF shares 92.9% amino acid (aa) sequence identity with mouse HLTF. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects DNA-dependent ATPase/E3 ubiquitin-protein ligase HLTF in human, mouse and rat samples and is validated for Western blot and ICC/IF. The predicted molecular weight is 113.9 kDa, and the supplier reports an observed band at about 114 kDa.
HLTF is a SWI/SNF-family DNA-dependent ATPase that also works as a RING E3 ubiquitin ligase. It polyubiquitinates PCNA to support error-free DNA damage tolerance and can remodel stalled replication forks, and it has additional roles in chromatin regulation. Its gene is often silenced by methylation in colorectal and other cancers.
| Target | DNA-dependent ATPase/E3 ubiquitin-protein ligase HLTF (gene HLTF; UniProt Q14527, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 113.9 kDa |
| Observed MW | 114 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
Samples with a confirmed band (WB): human HeLa cells, human PANC-1 cells, human A549 cells, rat heart tissue, rat kidney tissue, mouse heart tissue, mouse kidney tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): A549 cells.
Immunogen
Recombinant human HLTF fragment (Ser911–Leu1009), expressed in E. coli. Sequence identity with the mouse ortholog: 92.9%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places DNA-dependent ATPase/E3 ubiquitin-protein ligase HLTF in the nucleus and chromosome. Tissue expression noted by UniProt (human): Expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
