| Field | Specification |
|---|---|
| Target | |
| Alternative names | Porphobilinogen deaminase; PBG-D; Hydroxymethylbilane synthase; HMBS; Pre-uroporphyrinogen synthase; HMBS; PBGD; UPS |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human HMBS recombinant protein (Position: N6-H361). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes porphobilinogen deaminase (gene HMBS) in human, mouse and rat samples and is validated for ELISA and Western blot. The predicted molecular weight is 39.3 kDa, and the supplier reports an observed band at about 45 kDa.
Porphobilinogen deaminase (HMBS, also called hydroxymethylbilane synthase) is the third enzyme of heme biosynthesis. It links four porphobilinogen units into the linear tetrapyrrole hydroxymethylbilane, building the chain on its own dipyrromethane cofactor. HMBS deficiency causes acute intermittent porphyria.
| Target | Porphobilinogen deaminase (gene HMBS; UniProt P08397, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 39.3 kDa |
| Observed MW | 45 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HEL cells, human K562 cells, human MCF-7 cells, human Caco-2 cells, rat spleen tissue, mouse spleen tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human HMBS fragment (Asn6–His361), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places porphobilinogen deaminase in the cytoplasm. Tissue expression noted by UniProt (human): Is ubiquitously expressed... The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
