| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Hepatocyte nuclear factor 1-beta;HNF-1-beta;HNF-1B;Homeoprotein LFB3;Transcription factor 2;TCF-2;Variant hepatic nuclear factor 1;vHNF1;HNF1B;TCF2; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human HNF1 beta |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-HNF1B antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone 18H22; isotype IgG; reactivity: Human. Reported application contexts include WB (as provided in the source record). Boster Bio Anti-HNF1 beta Rabbit Monoclonal Antibody catalog # M01270-1. Tested in WB application. This antibody reacts with Human.
Key elements and design rationale
- Target: HNF1B (Hepatocyte nuclear factor 1-beta).
- Antibody format: Monoclonal; clone 18H22; isotype IgG.
- Host: Rabbit.
- Species reactivity: Human (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
HNF1B (protein: Glycogen synthase kinase-3 beta (gsk3b)) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Transcription factor, probably binds to the inverted palindrome 5'-GTTAATNATTAAC-3'. Reported cellular localization context: Nucleus. Tissue expression notes (as provided): Preferentially expressed in melanomas. Some expression was found in dysplastic nevi. Not found in normal tissues nor in carcinomas. Normally expressed at low levels in quiescent adult melanocytes but overexpressed by proliferating neonatal melanocytes and during tumor growth.
Research relevance and current trends
- Research context keywords from the source record include: Diabetes,Epigenetics and Nuclear Signaling,Metabolism,Transcription.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
Workflow ideas (metafield): Validate HNF1B antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect HNF1B expression by Western blot in cell or tissue lysates, Compare relative HNF1B levels across experimental conditions (dose/time-course) using antibody-based readouts
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 70 kDa; calculated MW: 61324 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 70 kDa
- Cellular localization (provided): Nucleus.
- Tissue details (provided): Preferentially expressed in melanomas. Some expression was found in dysplastic nevi. Not found in normal tissues nor in carcinomas. Normally expressed at low levels in quiescent adult melanocytes but overexpressed by proliferating neonatal melanocytes and during tumor growth.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
