| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Homeobox protein SIX6;Homeodomain protein OPTX2;Optic homeobox 2;Sine oculis homeobox homolog 6;SIX6;OPTX2, SIX9; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E. coli-derived human SIX6 recombinant protein (Position: A188-I246). Human SIX6 shares 91.5% amino acid (aa) sequence identity with mouse SIX6. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of SIX6 in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-Homeobox protein SIX6 Antibody Picoband® catalog # A06502-1. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E. coli-derived human SIX6 recombinant protein (Position: A188-I246). Human SIX6 shares 91.5% amino acid (aa) sequence identity with mouse SIX6. (reported region: A188-I246).
- Molecular weight context: reported MW: 28 kDa; calculated MW: 27687 MW
- Reactivity: Human
- Applications: WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Homeobox protein SIX6. The protein encoded by this gene is a homeobox protein that is similar to the Drosophila 'sine oculis' gene product. This gene is found in a cluster of related genes on chromosome 14 and is thought to be involved in eye development. Defects in this gene are a cause of isolated microphthalmia with cataract type 2 (MCOPCT2). Functional note: May be involved in eye development. Reported localization: Nucleus . Expression/tissue context: Expressed in the developing and adult retina. Also expressed in the hypothalamic and the pituitary regions.
Research relevance and current trends
- Developmental Families: Researchers commonly examine how SIX6 relates to this theme using model systems and orthogonal readouts.
- Domain Families: Researchers commonly examine how SIX6 relates to this theme using model systems and orthogonal readouts.
- Epigenetics and Nuclear Signaling: Researchers commonly examine how SIX6 relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative SIX6 levels across conditions; band patterns may reflect isoforms and processing.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
