| Field | Specification |
|---|---|
| Target | |
| Alternative names | Homer protein homolog 3; Homer-3; HOMER3 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human HOMER3 recombinant protein (Position: R282-A360). Human HOMER3 shares 88.6% and 89.9% amino acid (aa) sequence identity with mouse and rat HOMER3, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 9G13 is a mouse monoclonal antibody that recognizes homer protein homolog 3 (gene HOMER3) in human samples and is validated for Western blot and flow cytometry. The predicted molecular weight is 39.8 kDa, and the supplier reports an observed band at about 45 kDa.
Homer 3 (HOMER3) is a member of the Homer family of EVH1-domain scaffolding proteins that link metabotropic glutamate receptors, IP3 receptors and Shank proteins. It is highly expressed in cerebellar Purkinje cells and is studied in synaptic signaling and Ca2+ regulation.
| Target | Homer protein homolog 3 (gene HOMER3; UniProt Q9NSC5, human) |
|---|---|
| Clone | 9G13 |
| Host / Clonality / Isotype | Mouse / Monoclonal / Mouse IgG2a |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 39.8 kDa |
| Observed MW | 45 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human U-87MG tissue, human HeLa cells, human Caco-2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with a confirmed signal (flow cytometry): U937 cells.
Immunogen
Recombinant human HOMER3 fragment (Arg282–Ala360), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 88.6% and 89.9%, respectively.
Reactivity Notes
The supplier lists reactivity with human. UniProt places homer protein homolog 3 in the cytoplasm, postsynaptic density and synapse. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
