| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Homer protein homolog 3; Homer-3; HOMER3 |
| Cellular Localization | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived human HOMER3 recombinant protein (Position: R282-A360). Human HOMER3 shares 88.6% and 89.9% amino acid (aa) sequence identity with mouse and rat HOMER3, respectively. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-HOMER3 Antibody Picoband® (monoclonal, 9G13) is an antibody for HOMER3 detection raised in Mouse (Monoclonal, clone Clone: 9G13, Mouse IgG2a), with reported reactivity: Human. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: HOMER3 (homer scaffold protein 3); UniProt: Q9NSC5
- Antibody format: Mouse, Monoclonal, clone Clone: 9G13, Mouse IgG2a
- Molecular weight: 45 kDa
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-HOMER3 Antibody Picoband® (monoclonal, 9G13) catalog # M09145.
Biological background
Biological context: Postsynaptic density scaffolding protein. Binds and cross-links cytoplasmic regions of GRM1, GRM5, ITPR1, DNM3, RYR1, RYR2, SHANK1 and SHANK3. By physically linking GRM1 and GRM5 with ER-associated ITPR1 receptors, it aids the coupling of surface receptors to intracellular calcium release. Isoforms can be differently regulated and may play an important role in maintaining the plasticity at glutamatergic synapses. Negatively regulates T cell activation by inhibiting the calcineurin-NFAT pathway. Acts by competing with calcineurin/PPP3CA for NFAT protein binding, hence preventing NFAT activation by PPP3CA
Expression and localization notes: cellular localization: postsynaptic density; Cytoplasm; synapse, tissue context: Ubiquitously expressed with highest levels in spleen, thymus and immature brain..
Common research applications
- Western blotting (WB): Compare HOMER3 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of HOMER3 in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify HOMER3-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Specificity: No cross reactivity with other proteins.
- Background: Homer protein homolog 3 is a protein that in humans is encoded by the HOMER3 gene. This gene encodes a member of the HOMER family of postsynaptic density scaffolding proteins that share a similar domain structure consisting of an N-terminal Enabled/vasodilator-stimulated phosphoprotein homology 1 domain which mediates protein-protein interactions, and a carboxy-terminal coiled-coil domain and two leucine zipper motifs that are involved in self-oligomerization. The encoded protein binds numerous other proteins including group I metabotropic glutamate receptors, inositol 1,4,5-trisphosphate receptors and amyloid precursor proteins and has been implicated in diverse biological functions such as neuronal signaling, T-cell activation and trafficking of amyloid beta peptides.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: postsynaptic density; Cytoplasm; synapse
- Tissue details: Ubiquitously expressed with highest levels in spleen, thymus and immature brain.
- Research category: Chaperones,Protein Trafficking,Signal Transduction
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
