| Field | Specification |
|---|---|
| Target | |
| Alternative names | Homeobox protein Hox-A4; Homeobox protein Hox-1.4; Homeobox protein Hox-1D; HOXA4; HOX1D |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human HOXA4, which shares 72.2% and 61.1% amino acid (aa) sequence identity with mouse and rat HOXA4, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects homeobox protein Hox-A4 (gene HOXA4) in human samples and is validated for Western blot. The predicted molecular weight is 34.5 kDa, and the supplier reports an observed band at about 36 kDa.
HOXA4 is a homeobox transcription factor of the HOXA cluster that contributes to positional identity along the anterior-posterior axis. It helps pattern the cervical and upper thoracic region during development, and it is studied in development and in hematopoietic and cancer cells.
| Target | Homeobox protein Hox-A4 (gene HOXA4; UniProt Q00056, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 34.5 kDa |
| Observed MW | 36 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL/ml (Human) |
|---|
Samples with a confirmed band (WB): human SW620 cells, human 293T cells, human HepG2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide from the middle region of human HOXA4. Sequence identity with the mouse and rat orthologs: 72.2% and 61.1%, respectively.
Reactivity Notes
The supplier lists reactivity with human. UniProt places homeobox protein Hox-A4 in the nucleus. Tissue expression noted by UniProt (human): Embryonic nervous system. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
