| Field | Specification |
|---|---|
| Target | |
| Alternative names | Homeobox protein Hox-A9;Homeobox protein Hox-1G;HOXA9;HOX1G; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human HOXA9 recombinant protein (Position: M1-R204). Human HOXA9 shares 98% amino acid (aa) sequence identity with mouse HOXA9. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects homeobox protein Hox-A9 (gene HOXA9) in human and rat samples and is validated for Western blot. The predicted molecular weight is 30.2 kDa, and the supplier reports an observed band at about 30 kDa.
HOXA9 is a homeobox transcription factor that, like other HOX proteins, helps set positional identity in the embryo. In adults it is highly expressed in hematopoietic stem and progenitor cells, where it supports self-renewal. Its overexpression, often with MEIS1, is a hallmark of MLL-rearranged and other acute leukemias, and it also regulates endothelial genes.
| Target | Homeobox protein Hox-A9 (gene HOXA9; UniProt P31269, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 30.2 kDa |
| Observed MW | 30 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Rat) |
|---|
Samples with a confirmed band (WB): rat testis tissue, HepG2 cells, HEPA cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Recombinant human HOXA9 fragment (Met1–Arg204), expressed in E. coli. Sequence identity with the mouse ortholog: 98%.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places homeobox protein Hox-A9 in the nucleus and cytoplasm. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Qian Li et al. (2017) Effects of celecoxib on cell apoptosis and Fas, FasL and Bcl‑2 expression in a BGC‑823 human gastric cancer cell line. Experimental and Therapeutic Medicine. 10.3892/etm.2017.4769
