| Field | Specification |
|---|---|
| Target | |
| Alternative names | Homeobox protein Hox-B9;Homeobox protein Hox-2.5;Homeobox protein Hox-2E;HOXB9;HOX2E; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human HOXB9 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone DDG-8 is a rabbit monoclonal antibody that recognizes homeobox protein Hox-B9 (gene HOXB9) in human samples and is validated for Western blot, ICC/IF and immunoprecipitation. The predicted molecular weight is 28.1 kDa, and the supplier reports an observed band at about 28 kDa.
HOXB9 is a posterior homeobox transcription factor of the HOXB cluster that helps pattern the embryo along its head-to-tail axis, including thoracic skeletal elements. It is also studied as a regulator of angiogenesis-related and growth factor genes in tumor cells.
| Target | Homeobox protein Hox-B9 (gene HOXB9; UniProt P17482, human) |
|---|---|
| Clone | DDG-8 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 28.1 kDa |
| Observed MW | 28 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
Samples with a confirmed band (WB): MCF-7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human HOXB9.
Reactivity Notes
The supplier lists reactivity with human. UniProt places homeobox protein Hox-B9 in the nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
