| Field | Specification |
|---|---|
| Target | |
| Alternative names | Hypoxanthine-guanine phosphoribosyltransferase;HGPRT;HGPRTase;2.4.2.8;HPRT1;HPRT; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human HPRT recombinant protein (Position: A2-S110). Human HPRT shares 98.1% and 96.3% amino acid (aa) sequence identity with mouse and rat HPRT, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects hypoxanthine-guanine phosphoribosyltransferase (gene HPRT1) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 24.6 kDa, and the supplier reports an observed band at about 25 kDa.
Hypoxanthine-guanine phosphoribosyltransferase (HPRT1) is the key enzyme of the purine salvage pathway, recycling hypoxanthine and guanine into IMP and GMP. Complete loss of HPRT1 causes Lesch-Nyhan syndrome, and because it is steadily expressed it is also a common reference gene in expression studies.
| Target | Hypoxanthine-guanine phosphoribosyltransferase (gene HPRT1; UniProt P00492, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 24.6 kDa |
| Observed MW | 25 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|
Samples with a confirmed band (WB): human HeLa cells, human Colo320 cells, human HepG2 cells, human 293T cells, rat brain tissue, rat spleen tissue, mouse brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human HPRT fragment (Ala2–Ser110), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 98.1% and 96.3%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places hypoxanthine-guanine phosphoribosyltransferase in the cytoplasm. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Shulan Qi et al. (2024) Electrochemical response mechanism of DNA damaged cells: DNA damage repair and purine metabolism activation. Bioelectrochemistry. 10.1016/j.bioelechem.2024.108832
