| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Mitotic checkpoint serine/threonine-protein kinase BUB1 beta; MAD3/BUB1-related protein kinase; Hbubr1; Mitotic checkpoint kinase MAD3L; Protein SSK1; BUB1B; BUBR1, MAD3L, SSK1 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human HSD11B1 recombinant protein (Position: E30-K292). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-HSD11B1 Antibody Picoband® is an antibody for HSD11B1 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: HSD11B1 (BUB1 mitotic checkpoint serine/threonine kinase B); UniProt: P28845
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 36 kDa
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-HSD11B1 Antibody Picoband® catalog # A01565.
Biological background
Biological context: Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE. Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. May play a role for tumor suppression.
Expression and localization notes: cellular localization: Membrane; Colocalizes with CACNA1A in lipid raft fractions., tissue context: Highly expressed in thymus followed by spleen. Preferentially expressed in tissues with a high mitotic index..
Common research applications
- Western blotting (WB): Compare HSD11B1 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of HSD11B1 in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify HSD11B1-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: 11beta-hydroxysteroid dehydrogenase type 1 is an NADPH-dependent enzyme highly expressed in key metabolic tissues including liver, adipose tissue, and the central nervous system. In these tissues, HSD11B1 reduces cortisone to the active hormone cortisol that activates glucocorticoid receptors. It is inhibited by carbenoxolone, a drug typically used in the treatment of peptic ulcers. Tannin et al.(1991) localized the HSD11B1 gene to chromosome 1. The localization was confirmed by isolating the gene from a chromosome 1-specific library using the cDNA as a probe. Schutte et al.(2000) mapped the HSD11B1 gene to 1q32-q41.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Membrane; Colocalizes with CACNA1A in lipid raft fractions.
- Tissue details: Highly expressed in thymus followed by spleen. Preferentially expressed in tissues with a high mitotic index.
- Research category: Apoptosis,Associated Proteins,Calcium Channels,Calcium Signaling,Cell Biology,Intracellular,Neuroscience,Neurotransmission,Signal Transduction,Signaling Pathway
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
