| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Corticosteroid 11-beta-dehydrogenase isozyme 1;1.1.1.146;11-beta-hydroxysteroid dehydrogenase 1;11-DH;11-beta-HSD1;Short chain dehydrogenase/reductase family 26C member 1;HSD11B1;HSD11, HSD11L, SDR26C1; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human HSD11B1, different from the related mouse sequence by two amino acids, and from the related rat sequence by four amino acids. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-HSD11B1 Antibody Picoband® is an antibody targeting HSD11B1. Common applications include WB, IHC, Flow Cytometry, ELISA. Key specifications include host: Rabbit; clonality: Polyclonal; isotype: Rabbit IgG; reactivity: Human,Monkey,Mouse,Rat; observed MW: 36 kDa; calculated MW: 32401 MW.
Boster Bio Anti-HSD11B1 Antibody catalog # PA1372-1. Tested in IHC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Target: HSD11B1 — Corticosteroid 11-beta-dehydrogenase isozyme 1
- Antibody format: Host: Rabbit; Clonality: Polyclonal; Isotype: Rabbit IgG
- Species reactivity: Human,Monkey,Mouse,Rat
- Molecular weight guidance: Observed: 36 kDa; Calculated: 32401 MW
Specificity note: No cross reactivity with other proteins.
Biological background
Protein function (datasheet): Catalyzes reversibly the conversion of cortisol to the inactive metabolite cortisone. Catalyzes reversibly the conversion of 7-ketocholesterol to 7-beta-hydroxycholesterol. In intact cells, the reaction runs only in one ion, from 7- ketocholesterol to 7-beta-hydroxycholesterol (By similarity). .
Scientific background (datasheet): 11beta-hydroxysteroid dehydrogenase type 1 is an NADPH-dependent enzyme highly expressed in key metabolic tissues including liver, adipose tissue, and the central nervous system. In these tissues, HSD11B1 reduces cortisone to the active hormone cortisol that activates glucocorticoid receptors. It is inhibited by carbenoxolone, a drug typically used in the treatment of peptic ulcers. Tannin et al. (1991) localized the HSD11B1 gene to chromosome 1. The localization was confirmed by isolating the gene from a chromosome 1-specific library using the cDNA as a probe. Schutte et al. (2000) mapped the HSD11B1 gene to 1q32-q41.
Cellular localization (datasheet): Endoplasmic reticulum membrane ; Single-pass type II membrane protein .
Tissue details (datasheet): Widely expressed. Highest expression in liver.
Sequence similarities (datasheet): Belongs to the connexin family. Beta-type (group I) subfamily.
Research relevance and current trends
- Commonly studied in contexts related to Endocrine Metabolism,Hormone Biosynthesis,Lipid and Lipoprotein Metabolism,Lipid Metabolism,Metabolic Signaling Pathway,Metabolic Signaling Pathways,Metabolism,Metabolism of Lipids and Lipoproteins,Mitochondrial,Mitochondrial Markers,Mitochondrial Metabolism,Pathways and Processes,Signal Transduction.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- ELISA: Measure target abundance in compatible matrices using a standard-curve readout; ensure dilution linearity and appropriate controls.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
- Flow cytometry: Quantify target-positive populations in single-cell suspensions; pair with viability and isotype/FMO controls conceptually.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Cross-reactivity (datasheet): No cross-reactivity with other proteins
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a polyclonal antibody, this reagent may recognize multiple epitopes on the target, which can improve detection robustness but may require careful specificity controls.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
