| Field | Specification |
|---|---|
| Target | |
| Alternative names | Heat shock factor protein 1; HSF 1; Heat shock transcription factor 1; HSTF 1; HSF1; HSTF1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human HSF1, identical to the related mouse and rat sequences. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects heat shock factor protein 1 (gene HSF1) in human samples and is validated for Western blot, IHC and flow cytometry. The predicted molecular weight is 57.3 kDa, and the supplier reports an observed band at about 80 kDa.
Heat shock factor 1 (HSF1) is the master transcription factor of the heat shock response. Under proteotoxic stress it forms trimers, binds heat shock elements and switches on genes for chaperones such as HSP70 and HSP90, which protect cells from misfolded proteins. HSF1 is studied in protein homeostasis, aging, neurodegeneration and cancer.
| Target | Heat shock factor protein 1 (gene HSF1; UniProt Q00613, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 57.3 kDa |
| Observed MW | 80 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.25 µg/mL/ml (Human) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL/ml (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/mL/1×106 cells (Human) |
Samples with a confirmed band (WB): human HeLa cells, human K562 cells, human MCF-7 cells, human MOLT-4 cells, human 293T cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.25 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human esophageal squamous carcinoma tissue, human lung cancer tissue, human squamous cells carcinoma of cervix tissue, human tonsil tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with a confirmed signal (flow cytometry): MCF-7 cells.
Immunogen
Synthetic peptide from the C-terminal region of human HSF1. The peptide sequence is conserved in mouse and rat.
Reactivity Notes
The supplier lists reactivity with human. UniProt places heat shock factor protein 1 in the nucleus, cytoplasm and chromosome. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yifan Peng-Winkler et al. (2022) Zinc supplementation prior to heat shock enhances HSP70 synthesis through HSF1 phosphorylation at serine 326 in human peripheral mononuclear cells. Food & Function. 10.1039/D2FO01406H
