| Field | Specification |
|---|---|
| Target | |
| Alternative names | Heat shock protein beta-1; HspB1; Growth-related 25 kDa protein; Heat shock 25 kDa protein; HSP 25; Heat shock 27 kDa protein; HSP 27; p25; Hspb1; Hsp25; Hsp27 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived mouse Hsp27 recombinant protein (Position: M1-K209). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets heat shock protein beta-1 (gene Hspb1) in mouse and rat samples and is validated for ELISA, IHC and Western blot. The predicted molecular weight is 23.0 kDa, and the supplier reports an observed band at about 27 kDa.
HSP27 (HSPB1) is a small heat shock protein that forms large oligomers and binds unfolding proteins to prevent their aggregation. Its phosphorylation by p38 MAPK-activated kinases regulates its assembly and roles in actin organization and resistance to apoptosis, and it is a common marker of cellular stress. HSPB1 mutations cause inherited peripheral neuropathy.
| Target | Heat shock protein beta-1 (gene Hspb1; UniProt P14602, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 23.0 kDa |
| Observed MW | 27 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): rat heart tissue, rat stomach tissue, mouse heart tissue, mouse stomach tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): rat lung tissue, rat small intestine tissue, mouse small intestine tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Immunogen
Recombinant mouse Hsp27 fragment (Met1–Lys209), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt places heat shock protein beta-1 in the cytoplasm and nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
ZHENG Chao-xu et al. (2011) Expression of heat shock protein 27 in the esophageal tissue of rats with reflux esophagitis. Chinese Medical Journal. 10.3760/cma.j.issn.0366-6999.2011.15.018
Wang Zhi et al. (2009) Effects of mitogen-activated protein kinase signal pathway on heat shock protein 27 expression in human lens epithelial cells exposed to sodium salicylate in vitro. Journal of Huazhong University of Science and Technology-Medical Sciences. 10.1007/s11596-009-0323-x
