| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | 60 kDa heat shock protein, mitochondrial;60 kDa chaperonin;Chaperonin 60;CPN60;HSP-65;Heat shock protein 60;HSP-60;Hsp60;Mitochondrial matrix protein P1;Hspd1;Hsp60; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | Recombinant human heat shock protein 60 (HSP60) |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-HSP60 Hspd1 Antibody (Monoclonal, LK1) is an antibody targeting HSPD1. Common applications include WB, IHC. Key specifications include host: Mouse; clonality: Monoclonal; clone: Clone: LK1; isotype: Mouse IgG1; reactivity: Chicken,Human,Mouse,Rat; observed MW: 65 kDa; calculated MW: 60955 MW.
Boster Bio Anti-HSP60 Hspd1 Antibody (Monoclonal, LK1) catalog # MA1049. Tested in IHC, WB applications. This antibody reacts with Chicken, Human, Mouse, Rat.
Key elements and design rationale
- Target: HSPD1 — 60 kDa heat shock protein, mitochondrial
- Antibody format: Host: Mouse; Clonality: Monoclonal; Clone: Clone: LK1; Isotype: Mouse IgG1
- Species reactivity: Chicken,Human,Mouse,Rat
- Molecular weight guidance: Observed: 65 kDa; Calculated: 60955 MW
Specificity note: No cross reactivity with other proteins.
Biological background
Protein function (datasheet): Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix.
Scientific background (datasheet): Heat shock 60KD protein (HSP60) is a member of the chaperonin class of protein factors and the nuclear encoded mitochondrial HSP60 is required for the assembly into oligomeric complexes of proteins imported into the mitochondrial matrix. HSP60 is linked head to head comprising approximately 17 kb and consist of 12 exons. HSP60 is a self-molecule, it can downregulate adaptive immune responses by upregulating Tregs innately through TLR2 signaling.
Cellular localization (datasheet): Mitochondrion matrix .
Tissue details (datasheet): Expressed in a variety of tissues. High levels in lung, adrenal, xiphoid, adipose tissue, heart and striated and smooth muscle, lower levels in the CNS. Adult levels are much higher in the slow-twitch soleus muscle than in the fast-twitch rectus femoris and extensor digitorum muscles. .
Sequence similarities (datasheet): Belongs to the chaperonin (HSP60) family.
Research relevance and current trends
- Commonly studied in contexts related to Chaperones,Heat Shock Proteins,Mitochondria,Organelles,Protein Trafficking,Signal Transduction,Subcellular Markers,Tags & Cell Markers.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Cross-reactivity (datasheet): No cross-reactivity with other proteins
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
