| Field | Specification |
|---|---|
| Target | |
| Alternative names | Heat shock 70 kDa protein 1A ;Heat shock 70 kDa protein 1;HSP70-1 ;HSP70.1;HSPA1A;HSPA1, HSX70; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human Hsp70, different from the related mouse sequence by four amino acids and from the related rat sequence by three amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects heat shock 70 kDa protein 1A (gene HSPA1A) in human, mouse and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 70.1 kDa, and the supplier reports an observed band at about 70 kDa.
HSP70-1A (HSPA1A) is one of the major stress-inducible HSP70 chaperones. It uses ATP-driven binding and release of exposed hydrophobic segments to refold damaged proteins, prevent aggregation and direct unsalvageable proteins to degradation. Its strong induction by heat and other stresses makes it a widely used marker of the cellular stress response.
| Target | Heat shock 70 kDa protein 1A (gene HSPA1A; UniProt P0DMV8, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg thimerosal; 0.01 mg NaN3; plus stabilizers |
| Calculated MW | 70.1 kDa |
| Observed MW | 70 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human) |
| IHC (frozen sections) | 0.5–1 µg/mL (Rat) |
| Immunocytochemistry / Immunofluorescence | 0.5–1 µg/mL (Human) |
Samples with a confirmed band (WB): human HeLa cells, human HepG2 cells, human A431 cells, human MCF-7 cells, human A549 cells, human PC-3 cells, human 293T cells, human K562 cells, rat liver tissue, rat kidney tissue, rat brain tissue, rat RH35 cells, mouse liver tissue, mouse kidney tissue, mouse brain tissue, mouse NIH/3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human intestinal cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Samples with confirmed staining (IHC): rat intestine tissue.
Samples with confirmed staining (ICC/IF): A549 cells.
Immunogen
Synthetic peptide from the C-terminal region of human hsp70. It differs from the mouse sequence at four residues.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes heat shock 70 kDa protein 1A as secreted and also located in the cytoplasm and nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Xue Guan et al. (2019) circPUM1 Promotes Tumorigenesis and Progression of Ovarian Cancer by Sponging miR-615-5p and miR-6753-5p. Molecular Therapy-Nucleic Acids. 10.1016/j.omtn.2019.09.032
Qianhui Zhang et al. (2018) Activation of transient receptor potential vanilloid 3 channel (TRPV3) aggravated pathological cardiac hypertrophy via calcineurin/NFATc3 pathway in rats. Journal of Cellular and Molecular Medicine. 10.1111/jcmm.13880
Li Li et al. (2017) Expression of Hsp70 reveals significant differences between fin regeneration and inflammation in Paramisgurnus dabryanus. Fish & Shellfish Immunology. 10.1016/j.fsi.2017.03.024
