| Field | Specification |
|---|---|
| Target | |
| Alternative names | Heat shock protein HSP 90-beta;HSP 90;Heat shock 84 kDa;HSP 84;HSP84;HSP90AB1;HSP90B, HSPC2, HSPCB; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Hsp90 beta |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone CCC-8 is a rabbit monoclonal antibody that recognizes heat shock protein HSP 90-beta (gene HSP90AB1) in human, mouse and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 83.3 kDa, and the supplier reports an observed band at about 90 kDa.
HSP90β (HSP90AB1) is the constitutively expressed form of the HSP90 chaperone. It uses ATP-dependent conformational cycles and co-chaperones to support folding, stability and activity of many client proteins, including kinases and steroid hormone receptors, and it is studied in protein homeostasis, signaling and cancer.
| Target | Heat shock protein HSP 90-beta (gene HSP90AB1; UniProt P08238, human) |
|---|---|
| Clone | CCC-8 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 83.3 kDa |
| Observed MW | 90 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): human HeLa cells, human Raji cells, human Jurkat cells, human HepG2 cells, rat brain tissue, rat heart tissue, mouse brain tissue, mouse heart tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Samples with confirmed staining (IHC): human kidney tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human hsp90 beta.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes heat shock protein HSP 90-beta as secreted and also located in the cytoplasm, melanosome and nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Xiaodi Liang et al. (2021) Staufen1 unwinds the secondary structure and facilitates the translation of fatty acid binding protein 4 mRNA during adipogenesis. Adipocyte. 10.1080/21623945.2021.1948165
Chen Xiao-Fang et al. (2023) PRM-based quantitative proteomics analysis of altered HSP abundance in villi and decidua of patients with early missed abortion. Proteome Science. 10.1186/s12953-023-00213-w
