| Field | Specification |
|---|---|
| Target | |
| Alternative names | Heat shock protein beta-8;HspB8;Alpha-crystallin C chain;E2-induced gene 1 protein;Protein kinase H11;Small stress protein-like protein HSP22;HSPB8;CRYAC, E2IG1, HSP22;PP1629; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human HSPB8/HSP22 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone IOD-8 is a rabbit monoclonal antibody that recognizes heat shock protein beta-8 (gene HSPB8) in human, mouse and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 21.6 kDa, and the supplier reports an observed band at about 22 kDa.
HSPB8 (HSP22) is a small heat shock protein that works with BAG3 and HSP70 in chaperone-assisted selective autophagy, which clears damaged proteins, especially in muscle and neurons. HSPB8 mutations cause forms of inherited neuropathy and myopathy.
| Target | Heat shock protein beta-8 (gene HSPB8; UniProt Q9UJY1, human) |
|---|---|
| Clone | IOD-8 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 21.6 kDa |
| Observed MW | 22 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): human fetal heart.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human HSPB8/HSP22.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places heat shock protein beta-8 in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): Predominantly expressed in skeletal muscle and heart. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
