| Field | Specification |
|---|---|
| Target | |
| Alternative names | Serine protease HTRA3;3.4.21.-;High-temperature requirement factor A3;Pregnancy-related serine protease;HTRA3;PRSP; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human HtrA3, different from the related mouse sequence by four amino acids, and from the related rat sequence by three amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects serine protease HTRA3 in human samples and is validated for Western blot. The predicted molecular weight is 48.6 kDa, and the supplier reports an observed band at about 49 kDa.
HTRA3 is a secreted serine protease of the HtrA family that degrades extracellular matrix proteoglycans such as decorin and biglycan, as well as fibronectin. By acting on the matrix it can indirectly reduce TGF-β family signaling, and it is studied in placental development, ovarian biology and as a possible tumor suppressor.
| Target | Serine protease HTRA3 (gene HTRA3; UniProt P83110, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 48.6 kDa |
| Observed MW | 49 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|
Samples with a confirmed band (WB): PANC-1 cells, HepG2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Immunogen
Synthetic peptide from the C-terminal region of human htrA3. It differs from the mouse sequence at four residues and from the rat sequence at three residues.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes serine protease HTRA3 as a secreted protein. Tissue expression noted by UniProt (human): Widely expressed, with highest levels in both adult and fetal heart, ovary, uterus placenta, and bladder. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
