| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | B7-H1, PD-L1, Program Death Ligand |
| Conjugate | |
| Formulation | |
| Host | |
| Immunogen | Recombinant human extracellular CD274(B7-H1) |
| Product Type | |
| Shipping | |
| Storage |
Product Overview
Antibody ANC6H1 binds to recombinant CD274 and native cell surface CD274 expressed on stimulated PBMC.
CD274 (B7-H1, PD-L1, Programmed Death Ligand) is a member of the B7 family and is expressed on a variety of tissues including lymphoid cells. It plays an important role in regulation of T cell activation, and is involved in progression of cancer, arthritis and HIV infection (3). CD274 binding to its receptor CD279 (PD-1) on activated T cells can decrease proliferation. Conversely, ligation of CD279 on primed T cells can stimulate IL-10 production. High levels of CD274 present in Renal cell carcinoma is associated with poor prognosis (1) . Tumor expressed CD274 can increase apoptosis of tumor specific T cells resulting in better tumor cell survival (2). Gamma Interferon and PHA can up regulate CD274 expression on T cells.
Validated Applications
| Application | Recommended Dilution |
|---|---|
| Flow Cytometry (FC) | See datasheet |
| EIA | See datasheet |
Product Specifications
| Clone | ANC6H1 |
|---|---|
| Clonality | Monoclonal |
| Host Species | Mouse |
| Isotype | IgG1 kappaappa |
| Species Reactivity | Human |
| Conjugate | Biotin, FITC, PE, Unconjugated |
| Storage | Store at 2 - 5°C. Freeze/Thawing is not recommended. |
Immunogen / Antigen
Recombinant human extracellular CD274(B7-H1)
Safety & Handling
For Research Use Only (RUO). Not for diagnostic or therapeutic use. Handle as a potential biohazard in accordance with good laboratory practices.
Related Products
Explore complementary antibodies, ELISA kits, and detection reagents at BioHippo.
This antibody has been validated for FC, EIA. Always refer to the product datasheet for recommended dilution ranges and appropriate positive control samples for each application type.
Validated reactivity has been confirmed for Human. Cross-reactivity with additional species may exist based on sequence homology but has not been confirmed by the manufacturer. For unlisted species, we recommend running a preliminary validation experiment.
This antibody was raised in Mouse. For indirect detection (WB, IHC, IF), select an anti-Mouse secondary antibody that matches the isotype. For direct detection, check the variant selector for conjugated formats. Confirm secondary antibody compatibility before ordering.
Recombinant human extracellular CD274(B7-H1)
Please refer to the storage conditions on the product label and datasheet. As a general best practice: upon receipt, aliquot into single-use volumes to minimize freeze-thaw cycling, which can progressively reduce antibody performance and lead to aggregation.
Validated reactivity covers Human samples. Performance can vary across different cell lines and tissue types depending on target antigen expression levels and epitope accessibility. We recommend verifying target expression in your specific model system using a known positive control before proceeding to experimental samples.
BioHippo offers customization and value-added services for qualified research programs:
- Bulk Pricing: Volume discounts available for orders of 5 or more units. Request a quote through the Contact Us page.
- Custom Conjugation: Select antibody products are available for custom conjugation to fluorophores, biotin, HRP, or other detection labels upon request. Minimum quantities apply.
- Custom Formats: Lyophilized or concentrated formats may be available for select products. Inquire before ordering.
- Technical Support: Our scientific team is available to assist with application optimization, protocol troubleshooting, and experimental design.
For customization inquiries, please use the Contact Us page or reach out to our support team directly.
