| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | ICOSL, GL50, B7-H2 |
| Conjugate | |
| Formulation | |
| Host | |
| Immunogen | Human Raji cells, recombinant human CD275-muIg |
| Product Type | |
| Shipping | |
| Storage |
Product Overview
Antibody ANC4E3 binds to recombinant CD275 in EIA. It does not block the interaction between recombinant CD278 and CD275.
The inducible costimulator CD278 (ICOS) is similar to human CD28 (24% homology), and plays an analogous role in the T cell activation process. Unlike CD28, CD278 is only expressed on activated T cells. Secondary signaling through CD28 or CD278 results in discrete cytokine secretion profiles by the activated T cells(1). Engagement of CD152 (CTLA-4) anergizes cells costimulated with either CD28 and CD278(2). Signaling through CD278 is particularly important in progression of TH2 immune response (5). The receptor for human CD278(ICOS) is CD275(GL50,ICOS L), a member of the B7 family sharing ~20% homology with CD80 (B7-1) and CD86 (B7-2)(3). Two RNA splice variants exist for this molecule, differing only in the cytoplasmic domain(4). Blockade of the CD278-CD275 interaction in mice improves allograft survival(6) and reduces EAE(7).
Validated Applications
| Application | Recommended Dilution |
|---|---|
| EIA | See datasheet |
Product Specifications
| Clone | ANC4E3 |
|---|---|
| Clonality | Monoclonal |
| Host Species | Mouse |
| Isotype | IgG1 kappaappa |
| Species Reactivity | Human |
| Conjugate | Biotin, Unconjugated |
| Storage | Store at 2 - 5°C. Freeze/Thawing is not recommended. |
Immunogen / Antigen
Human Raji cells, recombinant human CD275-muIg
Safety & Handling
For Research Use Only (RUO). Not for diagnostic or therapeutic use. Handle as a potential biohazard in accordance with good laboratory practices.
Related Products
Explore complementary antibodies, ELISA kits, and detection reagents at BioHippo.
This antibody has been validated for EIA. Always refer to the product datasheet for recommended dilution ranges and appropriate positive control samples for each application type.
Validated reactivity has been confirmed for Human. Cross-reactivity with additional species may exist based on sequence homology but has not been confirmed by the manufacturer. For unlisted species, we recommend running a preliminary validation experiment.
This antibody was raised in Mouse. For indirect detection (WB, IHC, IF), select an anti-Mouse secondary antibody that matches the isotype. For direct detection, check the variant selector for conjugated formats. Confirm secondary antibody compatibility before ordering.
Human Raji cells, recombinant human CD275-muIg
Please refer to the storage conditions on the product label and datasheet. As a general best practice: upon receipt, aliquot into single-use volumes to minimize freeze-thaw cycling, which can progressively reduce antibody performance and lead to aggregation.
Validated reactivity covers Human samples. Performance can vary across different cell lines and tissue types depending on target antigen expression levels and epitope accessibility. We recommend verifying target expression in your specific model system using a known positive control before proceeding to experimental samples.
BioHippo offers customization and value-added services for qualified research programs:
- Bulk Pricing: Volume discounts available for orders of 5 or more units. Request a quote through the Contact Us page.
- Custom Conjugation: Select antibody products are available for custom conjugation to fluorophores, biotin, HRP, or other detection labels upon request. Minimum quantities apply.
- Custom Formats: Lyophilized or concentrated formats may be available for select products. Inquire before ordering.
- Technical Support: Our scientific team is available to assist with application optimization, protocol troubleshooting, and experimental design.
For customization inquiries, please use the Contact Us page or reach out to our support team directly.
