| Field | Specification |
|---|---|
| Alternative Names | ICOS, AILIM, anti-ICOS |
| Conjugate | |
| Formulation | |
| Host | |
| Immunogen | Human HPB-MLT cells, recombinant human CD278(ICOS)-muIg |
| Product Type | |
| Shipping | |
| Storage |
Product Overview
Antibody ANC6C6 binds to recombinant and cell surface CD278(ICOS)
The inducible costimulator CD278 (ICOS) is similar to human CD28 (24% homology), and plays an analogous role in the T cell activation process. Secondary signaling through CD28 or CD278 results in discrete cytokine secretion profiles by the activated T cells.1 Signaling by either molecule is effectively down regulated by CD152 (CTLA-4) engagement.2 Human CD275 (ICOSL, GL50, B7-H2) is a member of the B7 family sharing ~20% homology with CD80 (B7-1) and CD86 (B7-2), and has been shown to be the ligand for CD278 (ICOS).3 Two RNA splice variants exist for this molecule, differing only in the cytoplasmic domain. 4
Validated Applications
| Application | Recommended Dilution |
|---|---|
| Flow Cytometry (FC) | See datasheet |
| EIA | See datasheet |
Product Specifications
| Clone | ANC6C6-A3 |
|---|---|
| Clonality | Monoclonal |
| Host Species | Mouse |
| Isotype | IgG1 kappaappa |
| Species Reactivity | Human |
| Conjugate | APC, Biotin, FITC, PE, Unconjugated |
| Storage | Store at 2 - 5°C. Freeze/Thawing is not recommended. |
Immunogen / Antigen
Human HPB-MLT cells, recombinant human CD278(ICOS)-muIg
Functional Activity
Antibody ANC6C6 blocks binding of Recombinant CD275-muIg to CD278. It blocks CD28-superagonist/CD275(LICOS) mediated T cell proliferation in vitro(7).
Safety & Handling
For Research Use Only (RUO). Not for diagnostic or therapeutic use. Handle as a potential biohazard in accordance with good laboratory practices.
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This antibody has been validated for FC, EIA. Always refer to the product datasheet for recommended dilution ranges and appropriate positive control samples for each application type.
Validated reactivity has been confirmed for Human. Cross-reactivity with additional species may exist based on sequence homology but has not been confirmed by the manufacturer. For unlisted species, we recommend running a preliminary validation experiment.
This antibody was raised in Mouse. For indirect detection (WB, IHC, IF), select an anti-Mouse secondary antibody that matches the isotype. For direct detection, check the variant selector for conjugated formats. Confirm secondary antibody compatibility before ordering.
Human HPB-MLT cells, recombinant human CD278(ICOS)-muIg
Please refer to the storage conditions on the product label and datasheet. As a general best practice: upon receipt, aliquot into single-use volumes to minimize freeze-thaw cycling, which can progressively reduce antibody performance and lead to aggregation.
Validated reactivity covers Human samples. Performance can vary across different cell lines and tissue types depending on target antigen expression levels and epitope accessibility. We recommend verifying target expression in your specific model system using a known positive control before proceeding to experimental samples.
BioHippo offers customization and value-added services for qualified research programs:
- Bulk Pricing: Volume discounts available for orders of 5 or more units. Request a quote through the Contact Us page.
- Custom Conjugation: Select antibody products are available for custom conjugation to fluorophores, biotin, HRP, or other detection labels upon request. Minimum quantities apply.
- Custom Formats: Lyophilized or concentrated formats may be available for select products. Inquire before ordering.
- Technical Support: Our scientific team is available to assist with application optimization, protocol troubleshooting, and experimental design.
For customization inquiries, please use the Contact Us page or reach out to our support team directly.
Beier, K.C., R.A. Kroczek, et al. 2000, Eur J Immunol. 30(12):3707-3717.
Riley, J.L., C.H. June, et al. 2001, J. Immunol. 166: 4943-4948.
Ling, V., M. Collins, et al. 2000, J. Immunol. 164: 1653-1657.
Ling, V., M. Collins, et al. 2001, J. Immunol. 166: 7300-7308.
Tomoki Ito, Yong-Jun Liu, et al. (2008) Immunity 28: 870-880.
Hyung W Lim, Chang H Kim. (2007) J Immunol 179: 7448-7456.
S Weissmuller, Z Waibler, et al. (2012) Blood 119(26):6268-6277.
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