| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | H65-ricin A chain immunotoxin, H65-RTA |
| Clonality | |
| Expression System | |
| Form | Liquid |
| Host | |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Product Overview
Research Grade Zolimomab (HY046016) is a mouse monoclonal antibody detecting Lymphocyte antigen T1/Leu-1 in ELISA, Bioactivity: FACS, Functional assay, Research in vivo. Suitable for Human. Highlights- ●Multi-Application — Validated across multiple applications.
Specifications
| Host Species | Mouse |
|---|---|
| Species Reactivity | Human |
| Clonality | Monoclonal |
| Isotype | IgG1, nd |
| Target | Lymphocyte antigen T1/Leu-1, CD5, T-cell surface glycoprotein CD5, LEU1 |
| Purification | Protein A/G purified from cell culture supernatant. |
| Purity | >95% purity as determined by SDS-PAGE. |
| Form | Liquid |
| Storage Buffer | 0.01M PBS, pH 7.4. |
| Expression System | Mammalian Cells |
| UniProt | P06127 |
Validated Applications
| Application |
|---|
| ELISA |
| Flow Cytometry |
| Functional Assay |
| In Vivo Studies |
Scientific Background
T-cell surface glycoprotein CD5 (Lymphocyte antigen T1/Leu-1,CD5,T-cell surface glycoprotein CD5,LEU1) is a ~54 kDa protein. Lymphoid-specific receptor expressed by all T-cells and in a subset of B-cells known as B1a cells. Plays a role in the regulation of TCR and BCR signaling, thymocyte selection, T-cell effector differentiation and immune tolerance. Acts by interacting with several ligands expressed on B-cells such as CD5L or CD72 and thereby plays an important role in contact-mediated, T-dependent B-cell activation and in the maintenance of regulatory T and B-cell homeostasis. Functions as a negative regulator of TCR signaling during thymocyte development by associating with several signaling proteins including LCK, CD3Z chain, PI3K or CBL. Mechanistically, co-engagement of CD3 with CD5 enhances phosphorylated CBL recruitment leading to increased VAV1 phosphorylation and degradation.
Reactivity
This antibody shows reactivity with Human.
Safety & Handling
Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
1. Burgess, KE. et al. (1992) Proceedings of the National Academy of Sciences of the United States of America 89, 9311-5. PMID: 1384049
2. Osman, N. et al. (1992) European journal of immunology 22, 2995-3000. PMID: 1385158
3. Roa, NS. et al. (2013) Biochemical and biophysical research communications 432, 52-9. PMID: 23376399
4. Garaud, S. et al. (2018) Cellular & molecular immunology 15, 158-170. PMID: 27499044
