| Field | Specification |
|---|---|
| Alternative Names | Forkhead box P3, Scurfin, JM2, IPEX |
| Conjugate | |
| Formulation | |
| Host | |
| Immunogen | Recombinant human FOXP3 |
| Product Type | |
| Shipping | |
| Storage |
Product Overview
The FOXP3 molecule is a 50–55 kD transcription factor also known as IPEX, JM2, Forkhead box 3, and Scurfin. Defects in this gene can result in lethal autoimmune disease (2). When used with cell surface markers CD4 and CD25(IL-2R), FOXP3 is a useful marker for identifying T regulatory cells. Functionally, FOXP3 is thought to mediate oxidative phosphorylation, enabling Treg to function in a lower oxygen environment (3). In EIA, clone ANCFX2D7 binds to full length recombinant FOXP3 and to a FOXP3(R1-R3) construct (aa 1–198) but not to a FOXP3(R1) construct (aa 1-70), suggesting that its epitope…
Validated Applications
| Application | Recommended Dilution |
|---|---|
| Flow Cytometry (FC) | See datasheet |
| EIA | See datasheet |
Product Specifications
| Clone | ANCFX2D7 |
|---|---|
| Clonality | Monoclonal |
| Host Species | Mouse |
| Isotype | IgG1 kappaappa |
| Species Reactivity | Human |
| Conjugate | Biotin, FITC, PE, Unconjugated |
| Storage | Store at 2 - 5°C. Freeze/Thawing is not recommended. |
Immunogen / Antigen
Recombinant human FOXP3
Safety & Handling
For Research Use Only (RUO). Not for diagnostic or therapeutic use. Handle as a potential biohazard in accordance with good laboratory practices.
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This antibody has been validated for FC, EIA. Always refer to the product datasheet for recommended dilution ranges and appropriate positive control samples for each application type.
Validated reactivity has been confirmed for Human. Cross-reactivity with additional species may exist based on sequence homology but has not been confirmed by the manufacturer. For unlisted species, we recommend running a preliminary validation experiment.
This antibody was raised in Mouse. For indirect detection (WB, IHC, IF), select an anti-Mouse secondary antibody that matches the isotype. For direct detection, check the variant selector for conjugated formats. Confirm secondary antibody compatibility before ordering.
Recombinant human FOXP3
Please refer to the storage conditions on the product label and datasheet. As a general best practice: upon receipt, aliquot into single-use volumes to minimize freeze-thaw cycling, which can progressively reduce antibody performance and lead to aggregation.
Validated reactivity covers Human samples. Performance can vary across different cell lines and tissue types depending on target antigen expression levels and epitope accessibility. We recommend verifying target expression in your specific model system using a known positive control before proceeding to experimental samples.
BioHippo offers customization and value-added services for qualified research programs:
- Bulk Pricing: Volume discounts available for orders of 5 or more units. Request a quote through the Contact Us page.
- Custom Conjugation: Select antibody products are available for custom conjugation to fluorophores, biotin, HRP, or other detection labels upon request. Minimum quantities apply.
- Custom Formats: Lyophilized or concentrated formats may be available for select products. Inquire before ordering.
- Technical Support: Our scientific team is available to assist with application optimization, protocol troubleshooting, and experimental design.
For customization inquiries, please use the Contact Us page or reach out to our support team directly.
G Roncador, A H Banham, et al. (2005) Eur J Immunol 35(6): 1681-1691. PMID: 15902688.
S Hori, S Sakaguchi, (2004) Microbes and Infection 6(8): 745-751.
U H Beier, W W Hancock, et al. (2016) J Immunol 196(1 suppl 211.3).
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