| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | HLA-A, HLA-B, HLA-C, Major Histocompatibility complex |
| Conjugate | |
| Formulation | |
| Host | |
| Immunogen | Human HSB-2 T cell line |
| Product Type | |
| Shipping | |
| Storage |
Product Overview
Antibody 3F10 recognizes a monomorphic Class I epitope on the alpha chain (1). It has been reported to cross react with canine Class I (9,10).
Human MHC Class I (HLA-A, B & C) molecules are expressed on nearly all nucleated cells (2). MHC Class I molecules consist of heterodimers of a highly polymorphic transmembrane alpha chain and an invariant beta chain. MHC Class I molecules bind to endogenously synthesized peptides of about 8-10 amino acids which are presented to CD8+ T cells. Evidence indicates exogenously derived peptides are also presented by MHC Class I molecules (4).
Validated Applications
| Application | Recommended Dilution |
|---|---|
| Flow Cytometry (FC) | See datasheet |
| Immunohistochemistry (IHC) | See datasheet |
| EIA | See datasheet |
Product Specifications
| Clone | 3F10 |
|---|---|
| Clonality | Monoclonal |
| Host Species | Mouse |
| Isotype | IgG2 |
| Species Reactivity | Human |
| Conjugate | Biotin, FITC, PE, Unconjugated |
| Storage | Store at 2 - 5°C. Freeze/Thawing is not recommended. |
| Isotype Subclass | IgG2a |
Immunogen / Antigen
Human HSB-2 T cell line
Functional Activity
Antibody 3F10 (whole molecule) is cytotoxic in the presence of complement (1).
Safety & Handling
For Research Use Only (RUO). Not for diagnostic or therapeutic use. Handle as a potential biohazard in accordance with good laboratory practices.
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This antibody has been validated for FC, IHC, EIA. Always refer to the product datasheet for recommended dilution ranges and appropriate positive control samples for each application type.
Validated reactivity has been confirmed for Human. Cross-reactivity with additional species may exist based on sequence homology but has not been confirmed by the manufacturer. For unlisted species, we recommend running a preliminary validation experiment.
This antibody was raised in Mouse. For indirect detection (WB, IHC, IF), select an anti-Mouse secondary antibody that matches the isotype. For direct detection, check the variant selector for conjugated formats. Confirm secondary antibody compatibility before ordering.
Human HSB-2 T cell line
Please refer to the storage conditions on the product label and datasheet. As a general best practice: upon receipt, aliquot into single-use volumes to minimize freeze-thaw cycling, which can progressively reduce antibody performance and lead to aggregation.
Validated reactivity covers Human samples. Performance can vary across different cell lines and tissue types depending on target antigen expression levels and epitope accessibility. We recommend verifying target expression in your specific model system using a known positive control before proceeding to experimental samples.
BioHippo offers customization and value-added services for qualified research programs:
- Bulk Pricing: Volume discounts available for orders of 5 or more units. Request a quote through the Contact Us page.
- Custom Conjugation: Select antibody products are available for custom conjugation to fluorophores, biotin, HRP, or other detection labels upon request. Minimum quantities apply.
- Custom Formats: Lyophilized or concentrated formats may be available for select products. Inquire before ordering.
- Technical Support: Our scientific team is available to assist with application optimization, protocol troubleshooting, and experimental design.
For customization inquiries, please use the Contact Us page or reach out to our support team directly.
