| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | MUC-4, Ascites sialoglycoprotein 1, Ascites sialoglycoprotein 2, ASGP-1, ASGP-2, Testis mucin, MUC4, Ascites sialoglycoprotein, Pancreatic adenocarcinoma mucin, ASGP, Tracheobronchial mucin, Mucin-4 |
| Clonality | |
| Concentration | |
| Conjugate | |
| Form | Liquid |
| Host | |
| Isotype | |
| Product Type | |
| Reactivity | |
| Storage |
Overview
Anti-Human MUC4 Antibodies (8G7), APC is intended for detection of Human MUC4 Antibodies. Commonly used in ELISA, FCM applications. Typical workflows include Select staining controls in cells/tissues (isotype/KD/KO), Phenotype target-positive cells by flow cytometry in PBMCs/cell suspensions, Develop ELISA pairing using capture/detection antibodies and a standard curve, Optimize antibody dilution for staining in cells/tissues using a reference sample, QC lot-to-lot by comparing signal in a reference lysate or cell line. Relevant research areas: Molecular & Cellular Biology.
Also known as: Ascites sialoglycoprotein 1, Ascites sialoglycoprotein 2, Ascites sialoglycoprotein, Pancreatic adenocarcinoma mucin, Tracheobronchial mucin, Testis mucin, Mucin-4, ASGP-1, ASGP-2, MUC-4, MUC4, ASGP.
Reagent details: Host: Mouse; Clonality: Monoclonal; Isotype: IgG2a, kappa; Conjugate: APC; Format: Liquid; Reactivity: Human.
Target biology
Human MUC4 Antibodies (Ascites sialoglycoprotein 1) is a pathogen-derived antigen used to detect infection, antigen expression, or immune responses in research assays. Assay performance can depend on native conformation and sample preparation (e.g., denaturing vs native conditions). Common alternative names and symbols used in the literature include: Ascites sialoglycoprotein 2, Ascites sialoglycoprotein, Pancreatic adenocarcinoma mucin, Tracheobronchial mucin, Testis mucin, Mucin-4, ASGP-1, ASGP-2, MUC-4, MUC4, ASGP. Based on the annotated name/class, the antigen is most often associated with context-dependent localization, though this can vary by model system.
How this reagent helps
This antibody enables sensitive detection of the intended antigen in common research assays. For best performance, interpret signal in the context of appropriate controls and orthogonal validation when available.
Recommended experimental notes
- Titrate the antibody/reagent to optimize signal-to-noise for your specific sample type and detection system.
- Include appropriate controls (e.g., unstained, secondary-only where relevant, and biological positives/negatives).
- For flow cytometry, include Fc blocking when applicable, use a viability dye, and consider staining live cells for surface markers prior to fixation/permeabilization.
- For ELISA, optimize capture/detection pairing and run a standard curve in matrix-matched diluent when possible.
Storage
Store at 4°C for 12 months. Protect from light. Do not freeze. 0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300.
For research use only. Not for diagnostic procedures.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
