| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Potassium-transporting ATPase subunit beta;Gastric H (+)/K (+) ATPase subunit beta;Proton pump beta chain;ATP4B; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human ATP4B, different from the related mouse and rat sequences by five amino acids. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of ATP4B (Potassium-transporting ATPase subunit beta) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-Hydrogen Potassium ATPase Beta/ATP4B Antibody Picoband® catalog # A08719-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: A synthetic peptide corresponding to a sequence at the N-terminus of human ATP4B, different from the related mouse and rat sequences by five amino acids.
- Molecular weight context: reported MW: 60 kDa; calculated MW: 33367 MW
- Reactivity: Human,Mouse,Rat
- Applications: WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Potassium-transporting ATPase subunit beta; Potassium-transporting ATPase subunit beta. This gene encodes the beta subunit of the gastric H+, K+-ATPase. The protein encoded by this gene belongs to a family of P-type cation-transporting ATPases. The gastric H+, K+-ATPase is a heterodimer consisting of a high molecular weight catalytic alpha subunit and a smaller but heavily glycosylated beta subunit. This enzyme is a proton pump that catalyzes the hydrolysis of ATP coupled with the exchange of H (+) and K (+) ions across the plasma membrane. It is also responsible for gastric acid secretion. Functional note: Required for stabilization and maturation of the catalytic proton pump alpha subunit and may also involved in cell adhesion and establishing epithelial cell polarity. . Reported localization: Cell membrane; Single-pass type II membrane protein.
Research relevance and current trends
- ATPases: Researchers commonly examine how ATP4B (Potassium-transporting ATPase subunit beta) relates to this theme using model systems and orthogonal readouts.
- Cancer: Researchers commonly examine how ATP4B (Potassium-transporting ATPase subunit beta) relates to this theme using model systems and orthogonal readouts.
- Cofactors: Researchers commonly examine how ATP4B (Potassium-transporting ATPase subunit beta) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative ATP4B (Potassium-transporting ATPase subunit beta) levels across conditions; band patterns may reflect isoforms and processing.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
